Literature DB >> 6483622

A rapid deprotection procedure for phosphotriester DNA synthesis.

T P Patel, M A Chauncey, T A Millican, C C Bose, M A Eaton.   

Abstract

An equimolar solution of aldoxime and tetramethylguanidine at 70 degrees C removes both the base and phosphate protection from oligonucleotides prepared by solid phase phosphate triester technology. The rate of cleavage from the succinyl linkage commonly used for solid phase synthesis is also increased. The method is simpler, faster and more easily automated than existing methods.

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Year:  1984        PMID： 6483622      PMCID： PMC320121          DOI： 10.1093/nar/12.17.6853

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  3 in total

1.  Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.

Authors:  T P Patel; T A Millican; C C Bose; R C Titmas; G A Mock; M A Eaton
Journal:  Nucleic Acids Res       Date:  1982-09-25       Impact factor: 16.971

2.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

3.  Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl esters.

Authors:  C B Reese; L Zard
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971
  3 in total
  3 in total

1.  Modification of guanine bases by nucleoside phosphoramidite reagents during the solid phase synthesis of oligonucleotides.

Authors:  R T Pon; M J Damha; K K Ogilvie
Journal:  Nucleic Acids Res       Date:  1985-09-25       Impact factor: 16.971

2.  Highly efficient oligodeoxyribonucleotide synthesis using fully base protected phosphodiester building blocks carrying 2-(1-methylimidazol-2-yl) phenyl protection of the phosphate.

Authors:  B S Sproat; P Rider; B Beijer
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

3.  alpha-DNA II. Synthesis of unnatural alpha-anomeric oligodeoxyribonucleotides containing the four usual bases and study of their substrate activities for nucleases.

Authors:  F Morvan; B Rayner; J L Imbach; S Thenet; J R Bertrand; J Paoletti; C Malvy; C Paoletti
Journal:  Nucleic Acids Res       Date:  1987-04-24       Impact factor: 16.971
  3 in total

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