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Literature DB >> 6477510

Cobalt-ion chelate affinity chromatography for the purification of brain neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase.

Abstract

A method is described for the purification of neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase from monkey brain by utilizing Co2+ chelate affinity chromatography. The neutral enzyme, which selectively bound to the metal-ion chelate column, was elutable by Tris at pH 7.5 and gave over 80-fold purification in a single step with 100% recovery.

Entities: Chemical

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Year: 1984 PMID： 6477510 PMCID： PMC1144168 DOI： 10.1042/bj2220261

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. Metal chelate affinity chromatography, a new approach to protein fractionation.

Authors: J Porath; J Carlsson; I Olsson; G Belfrage
Journal: Nature Date: 1975-12-18 Impact factor: 49.962

2. The purification, properties and characterization of three forms of alpha-L-fucosidase from monkey brain.

Authors: T Alam; A S Balsubramanian
Journal: Biochim Biophys Acta Date: 1978-06-09

3. Separation, purification, comparative properties & subcellular localization of acid & neutral alpha-D-mannosidase of monkey brain.

Authors: R Mathur; A S Balasubramanian
Journal: Indian J Biochem Biophys Date: 1981-10 Impact factor: 1.918

Cobalt-ion chelate affinity chromatography for the purification of brain neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase.

1. Metal chelate affinity chromatography, a new approach to protein fractionation.

2. The purification, properties and characterization of three forms of alpha-L-fucosidase from monkey brain.

3. Separation, purification, comparative properties & subcellular localization of acid & neutral alpha-D-mannosidase of monkey brain.

4. The multiple forms of alpha-D-mannosidase in human plasma.

5. Multiple alpha-mannosidase activities in mammalian tissues as shown by metal-ion activation.

6. Characterization of human liver alpha-D-mannosidase purified by affinity chromatography.

7. Evidence for two distinct forms of mammalian beta-mannosidase.

8. Mannosidosis in Angus cattle. The enzymic defect.

9. Evidence for an alpha-mannosidase in endoplasmic reticulum of rat liver.

10. Caprine beta-mannosidosis. Inherited deficiency of beta-D-mannosidase.

1. Co2+-mediated time- and temperature-dependent activation of neutral alpha-D-mannosidase from monkey brain.

Review 2. Generation and degradation of free asparagine-linked glycans.

3. Purification of Pyoverdines of Pseudomonas fluorescens 2-79 by Copper-Chelate Chromatography.

4. Activation of cyclin-dependent kinase 4 (cdk4) by mouse MO15-associated kinase.