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Literature DB >> 6469724

Spectrophotometric characteristics and assay of pure pyronin Y.

Abstract

The spectrophotometric characteristics of analytically pure pyronin Y have been investigated. Addition of metal ions (Fe3+, Zn2+, and Mg2+) and of dextrin were shown not to influence the absorption characteristics. The composition of the solvent strongly influenced the value of the extinction coefficient. Aqueous ethanolic solutions with a content of about 50% ethanol gave higher epsilon-values than those found for more concentrated ethanol solutions. The difference can be explained by the existence of a solvent-solute complex in the less concentrated ethanol solutions. A new spectrophotometric assay is proposed using the epsilon-value 11.7 X 10(4) lmol-1 cm-1 found in aqueous ethanol (52%) as standard.

Entities: Chemical

Mesh：

Substances：

Year: 1984 PMID： 6469724 DOI： 10.1007/bf00495408

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

5 in total

4. [Romanowsky dyes and romanowsky-Giemsa effect. 1. Azure B, purity and content of dye samples, association (author's transl)].

Authors: E Zipfel; J R Grezes; W Seiffert; H W Zimmermann
Journal: Histochemistry Date: 1981

5. Purity of commercial non-certified European samples of Pyronin Y.

Authors: H Lyon; A P Andersen; I Andersen; P P Clausen; B Herold
Journal: Histochem J Date: 1982-07

5 in total

1. Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the methyl green-pyronin technique with the gallocyanin chromalum and Feulgen procedures using image cytometry.

Authors: E K Schulte; H O Lyon; P E Hoyer
Journal: Histochem J Date: 1992-06

2. Dye standards, Part II. 1: Pyronin Y (CI 45005). European Committee for Clinical Laboratory Standards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS).

Authors:
Journal: Histochem J Date: 1992-04

3. Nucleic acid staining with the methyl green-pyronin method. A comparison of the use of pure dyes and commercially available dyes.

Authors: A P Andersen; H Lyon; P Jakobsen; P E Høyer
Journal: Histochemistry Date: 1986

4. An investigation of new commercial samples of methyl green and pyronin Y.

Authors: H Lyon; P Jakobsen; P Høyer; A P Andersen
Journal: Histochem J Date: 1987 Jun-Jul

5. The correlation between uptake of methyl green and Feulgen staining intensity of cell nuclei. An image analysis study.

Authors: H Lyon; E Schulte; P E Hoyer
Journal: Histochem J Date: 1989-08

5 in total

Spectrophotometric characteristics and assay of pure pyronin Y.

1. Schiff-type reagents in cytochemistry. 2. Detection of primary amine dye impurities in pyronin B and pyronin Y(G).

2. Spectrophotometry of dyes. I. Methyl green. II. Pyronin.

3. The identification and purification of pyronin and rhodamine dyes.

4. [Romanowsky dyes and romanowsky-Giemsa effect. 1. Azure B, purity and content of dye samples, association (author's transl)].

5. Purity of commercial non-certified European samples of Pyronin Y.

1. Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the methyl green-pyronin technique with the gallocyanin chromalum and Feulgen procedures using image cytometry.

2. Dye standards, Part II. 1: Pyronin Y (CI 45005). European Committee for Clinical Laboratory Standards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS).

3. Nucleic acid staining with the methyl green-pyronin method. A comparison of the use of pure dyes and commercially available dyes.

4. An investigation of new commercial samples of methyl green and pyronin Y.

5. The correlation between uptake of methyl green and Feulgen staining intensity of cell nuclei. An image analysis study.