Literature DB >> 2444570

An investigation of new commercial samples of methyl green and pyronin Y.

H Lyon1, P Jakobsen, P Høyer, A P Andersen.   

Abstract

New commercial samples of Methyl Green (Gurr Certistain), Pyronine G (Gurr Certistain) and Pyronin Y (Polysciences) have been investigated using spectrophotometry, thin layer chromatography and nuclear magnetic resonance, in addition to standardized simultaneous and sequential staining methods using purified Ethyl Green and pure Pyronin Y as reference dyes. The Methyl Green was found to be Ethyl Green contaminated with Crystal Violet. It did not have any advantages compared with Ethyl Green supplied by American dye companies. The Pyronine G sample was Pyronin Y with a high dye content that gave good staining results when used with purified Ethyl Green. Pyronin Y from Polysciences was found to be essentially pure Pyronin Y.

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Year:  1987        PMID： 2444570     DOI： 10.1007/bf01680456

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  5 in total

1.  Standardized methyl green-pyronin Y procedures using pure dyes.

Authors:  P E Høyer; H Lyon; P Jakobsen; A P Andersen
Journal:  Histochem J       Date:  1986 Feb-Mar

2.  Nucleic acid staining with the methyl green-pyronin method. A comparison of the use of pure dyes and commercially available dyes.

Authors:  A P Andersen; H Lyon; P Jakobsen; P E Høyer
Journal:  Histochemistry       Date:  1986

Review 3.  The impurities of biological dyes: their detection, removal, occurrence and histological significance--a review.

Authors:  R W Horobin
Journal:  Histochem J       Date:  1969-02

4.  Spectrophotometric characteristics and assay of pure pyronin Y.

Authors:  P Jakobsen; H Lyon; S Treppendahl
Journal:  Histochemistry       Date:  1984

5.  Preparation and characterization of methyl green tetrafluoroborate.

Authors:  P Jakobsen; A P Andersen; H Lyon
Journal:  Histochemistry       Date:  1984
  5 in total
  2 in total

1.  Quantitative amplification of genomic DNA from histological tissue sections after staining with nuclear dyes and laser capture microdissection.

Authors:  T Ehrig; S A Abdulkadir; S M Dintzis; J Milbrandt; M A Watson
Journal:  J Mol Diagn       Date:  2001-02       Impact factor: 5.568

2.  The correlation between uptake of methyl green and Feulgen staining intensity of cell nuclei. An image analysis study.

Authors:  H Lyon; E Schulte; P E Hoyer
Journal:  Histochem J       Date:  1989-08
  2 in total

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