Literature DB >> 6459085

Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts.

W Y Hung, A Tourian.   

Abstract

The hypothesis that there is impaired endogenous synthesis of glucosamine 6-phosphate in Huntington's-chorea fibroblasts was tested by double labelling matched pairs of fibroblasts in culture with carrier-free H3 32PO4 and [U-14C]glucosamine. The [32P]UDP-N-acetyl[14C]glucosamine and [14C]glucosamine 6-[32P]phosphate of the cellular soluble fraction was isolated by charcoal column and paper chromatography. There is no quantitative difference in 32P but a significant difference in 14C in these two sugars in a ratio of approx. 1.5 for Huntington's-chorea fibroblasts compared with normal fibroblasts.

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Year:  1981        PMID： 6459085      PMCID： PMC1163021          DOI： 10.1042/bj1960495

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  Methods for the isolation of glycolytic intermediated by column chromatography with ion exchange resins.

Authors:  G R BARTLETT
Journal:  J Biol Chem       Date:  1959-03       Impact factor: 5.157

2.  Membrane abnormalities of Huntington's chorea fibroblasts in culture.

Authors:  A Tourian; W Y Hung
Journal:  Biochem Biophys Res Commun       Date:  1977-10-24       Impact factor: 3.575

3.  Glucosamine dependence of Huntington's chorea fibroblasts in culture.

Authors:  A Tourian; W Hung
Journal:  Biochem Biophys Res Commun       Date:  1976-05-23       Impact factor: 3.575

4.  Transport and metabolism of glucosamine by cultured Novikoff rat hepatoma cells and effects on nucleotide pools.

Authors:  P G Plagemann; J Erbe
Journal:  Cancer Res       Date:  1973-03       Impact factor: 12.701

5.  Glycoprotein synthesis in a temperature-sensitive Chinese hamster cell cycle mutant.

Authors:  A Tenner; J Zieg; I E Scheffler
Journal:  J Cell Physiol       Date:  1977-02       Impact factor: 6.384

6.  Cell culture in serum depleted of glycosidases by heating.

Authors:  R Bloch; B Betschart; M M Burger
Journal:  Exp Cell Res       Date:  1977-01       Impact factor: 3.905

7.  A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.

Authors:  W C Russell; C Newman; D H Williamson
Journal:  Nature       Date:  1975-02-06       Impact factor: 49.962

8.  Huntington's-chorea fibroblasts. Cellular protein glycosylation.

Authors:  W Y Hung; D E Mold; A Tourian
Journal:  Biochem J       Date:  1980-09-15       Impact factor: 3.857

9.  Kinetics of mucopolysaccharide and glycoprotein synthesis by chick embryo chondrocytes. Effect of D-glucose concentration in the culture medium.

Authors:  J J Kim; H E Conrad
Journal:  J Biol Chem       Date:  1976-10-25       Impact factor: 5.157
  9 in total
  1 in total

Review 1.  The current state of research with peripheral tissues in Huntington disease.

Authors:  G C Beverstock
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132
  1 in total

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