Literature DB >> 6449403

An easy method for the selection of restriction- and modification-deficient mutants of Escherichia coli K-12.

M Piechaczyk, P Jeanteur, J M Louarn.   

Abstract

An easy and rapid method for selecting restriction- and modification-defective mutants of Escherichia coli K-12 is described. This method employs selection of tetracycline resistant lysogens after infection with lambda::Tn10 phage and results in a high yield of spontaneous rk-mk- and rk-mk+ mutants.

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Year:  1980        PMID: 6449403     DOI: 10.1016/0378-1119(80)90098-0

Source DB:  PubMed          Journal:  Gene        ISSN: 0378-1119            Impact factor:   3.688


  3 in total

1.  Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome.

Authors:  M Jekel; W Wackernagel
Journal:  J Bacteriol       Date:  1994-03       Impact factor: 3.490

2.  Comparison of the regulatory regions of ilvGEDA operons from several enteric organisms.

Authors:  E Harms; J H Hsu; C S Subrahmanyam; H E Umbarger
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

3.  Isolation of insertion, deletion, and nonsense mutations of the uracil-DNA glycosylase (ung) gene of Escherichia coli K-12.

Authors:  B K Duncan
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

  3 in total

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