Literature DB >> 6445561

Molecular cloning of unintegrated Moloney mouse sarcoma virus DNA in bacteriophage lambda.

I M Verma, M H Lai, R A Bosselman, M A McKennett, H Fan, A Berns.   

Abstract

The covalently closed circular forms of unintegrated viral DNA obtained from cells infected with Moloney mouse sarcoma virus was cloned in bacteriophage lambda. The viral DNA was cleaved with restriction endonuclease HindIII and inserted in the unique HindIII site of lambda Charon 21A DNA. Recombinant clones containing virus-reactive DNA sequences were analyzed by restriction endonuclease mapping, R-loop formation, and infectivity assays. Two of eight genome-length recombinant clones characterized contained the large terminal repeat. Only the recombinant clones containing the large terminal repeat were able to induce focus formation in uninfected mouse fibroblasts.

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Year:  1980        PMID： 6445561      PMCID： PMC348589          DOI： 10.1073/pnas.77.4.1773

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  31 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

3.  Evidence for helper independent murine sarcoma virus. I. Segregation of replication-defective and transformation-defective viruses.

Authors:  J Ball; J A McCarter; S M Sunderland
Journal:  Virology       Date:  1973-11       Impact factor: 3.616

4.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

5.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

6.  Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors:  M W McDonell; M N Simon; F W Studier
Journal:  J Mol Biol       Date:  1977-02-15       Impact factor: 5.469

Review 7.  Structure of the intermediates leading to the integrated provirus.

Authors:  R A Weinberg
Journal:  Biochim Biophys Acta       Date:  1977-03-21

Review 8.  The reverse transcriptase.

Authors:  I M Verma
Journal:  Biochim Biophys Acta       Date:  1977-03-21

9.  An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.

Authors:  N D Stow; N M Wilkie
Journal:  J Gen Virol       Date:  1976-12       Impact factor: 3.891

10.  A heteroduplex study of the sequence relationships between the RNAs of M-MSV and M-MLV.

Authors:  S Hu; N Davidson
Journal:  Cell       Date:  1977-03       Impact factor: 41.582
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  22 in total

1.  Sequences upstream of c-mos(rat) that block RNA accumulation in mouse cells do not inhibit in vitro transcription.

Authors:  F A van der Hoorn; V Müller; L I Pizer
Journal:  Mol Cell Biol       Date:  1985-02       Impact factor: 4.272

2.  SV7, a molecular clone of Moloney murine sarcoma virus 349, transforms vascular endothelial cells.

Authors:  P H Yuen; C M Matherne; L M Molinari-Storey
Journal:  Am J Pathol       Date:  1991-12       Impact factor: 4.307

3.  Introduction of rat growth hormone gene into mouse fibroblasts via a retroviral DNA vector: expression and regulation.

Authors:  J Doehmer; M Barinaga; W Vale; M G Rosenfeld; I M Verma; R M Evans
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

4.  Long terminal repeat of murine retroviral DNAs: sequence analysis, host-proviral junctions, and preintegration site.

Authors:  C Van Beveren; E Rands; S K Chattopadhyay; D R Lowy; I M Verma
Journal:  J Virol       Date:  1982-02       Impact factor: 5.103

5.  Elements in the long terminal repeat of murine retroviruses enhance stable transformation by thymidine kinase gene.

Authors:  D J Jolly; A C Esty; S Subramani; T Friedmann; I M Verma
Journal:  Nucleic Acids Res       Date:  1983-03-25       Impact factor: 16.971

6.  Molecular cloning and characterization of a leukemia-inducing myeloproliferative sarcoma virus and two of its temperature-sensitive mutants.

Authors:  R Kollek; C Stocking; F Smadja-Joffe; W Ostertag
Journal:  J Virol       Date:  1984-06       Impact factor: 5.103

7.  FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA.

Authors:  T Curran; G Peters; C Van Beveren; N M Teich; I M Verma
Journal:  J Virol       Date:  1982-11       Impact factor: 5.103

8.  Structure of Moloney murine leukemia viral DNA: nucleotide sequence of the 5' long terminal repeat and adjacent cellular sequences.

Authors:  C Van Beveren; J G Goddard; A Berns; I M Verma
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

9.  Molecular cloning of unintegrated and a portion of integrated moloney murine leukemia viral DNA in bacteriophage lambda.

Authors:  A J Berns; M H Lai; R A Bosselman; M A McKennett; L T Bacheler; H Fan; E C Maandag; H V van der Putten; I M Verma
Journal:  J Virol       Date:  1980-10       Impact factor: 5.103

10.  Deletions of specific regions of the simian sarcoma-associated virus genome are found in defective viruses and in the simian sarcoma virus.

Authors:  E P Gelmann; E Petri; A Cetta; F Wong-Staal
Journal:  J Virol       Date:  1982-02       Impact factor: 5.103
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