Literature DB >> 6442109

Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes.

S S Twining.   

Abstract

A simple inexpensive sensitive protease assay was developed using soluble fluorescein isothiocyanate (FITC)-labeled casein. Casein was reacted with FITC to form the fluorescein thiocarbamoyl derivative. This substrate is cleaved by trypsin, chymotrypsin, elastase, subtilisin, and thermolysin in a linear time-dependent manner. These enzymes can be measured in the nanogram and subnanogram range using this assay. The assay is reproducible, has a low blank, and uses casein, which resembles natural substrates of most proteases.

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Year:  1984        PMID： 6442109     DOI： 10.1016/0003-2697(84)90553-0

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  106 in total

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2.  Calpain-PKC inter-relations in mouse hippocampus: a biochemical approach.

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3.  Ara12 subtilisin-like protease from Arabidopsis thaliana: purification, substrate specificity and tissue localization.

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4.  The autoproteolysis of Lactococcus lactis lactocepin III affects its specificity towards beta-casein.

Authors:  B Flambard; V Juillard
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

5.  Action of a cell wall proteinase from Lactococcus lactis subsp. cremoris SK11 on bovine alpha s1-casein.

Authors:  J R Reid; C H Moore; G G Midwinter; G G Pritchard
Journal:  Appl Microbiol Biotechnol       Date:  1991-05       Impact factor: 4.813

6.  The reactive serine residue of epidermolytic toxin A.

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Journal:  Biochem J       Date:  1990-07-15       Impact factor: 3.857

7.  Evidence that the major outer membrane protein of Chlamydia trachomatis is glycosylated.

Authors:  A F Swanson; C C Kuo
Journal:  Infect Immun       Date:  1991-06       Impact factor: 3.441

8.  Identification and characterization of a locus which regulates multiple functions in Pseudomonas tolaasii, the cause of brown blotch disease of Agaricus bisporus.

Authors:  S I Grewal; B Han; K Johnstone
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

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Authors:  Jiahn-Haur Liao; Yu-Ching Lin; Jowey Hsu; Alan Yueh-Luen Lee; Tse-An Chen; Chun-Hua Hsu; Jiun-Ly Chir; Kuo-Feng Hua; Tzu-Hua Wu; Li-Jenn Hong; Pei-Wen Yen; Arthur Chiou; Shih-Hsiung Wu
Journal:  Biophys J       Date:  2010-01-06       Impact factor: 4.033

10.  Autophagy in Tobacco Suspension-Cultured Cells in Response to Sucrose Starvation.

Authors:  Y. Moriyasu; Y. Ohsumi
Journal:  Plant Physiol       Date:  1996-08       Impact factor: 8.340
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