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Literature DB >> 6436145

One-step purification of hybrid proteins which have beta-galactosidase activity.

Abstract

A one-step purification method of hybrid proteins exhibiting beta-galactosidase activity, based on affinity chromatography in the presence of high salt concentration, is described. Starting from crude bacterial extracts, several milligrams of near-homogeneous proteins can be obtained in a few hours with an overall yield of 85 to 95%. The purified hybrid proteins can be used to obtain antibodies against the foreign portion of the protein fusion.

Entities: Chemical Gene

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Year: 1984 PMID： 6436145 DOI： 10.1016/0378-1119(84)90162-8

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

59 in total

1. Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2.

Authors: M Amemura; K Makino; H Shinagawa; A Nakata
Journal: J Bacteriol Date: 1990-11 Impact factor: 3.490

2. Rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases.

Authors: A Beuve; B Boesten; M Crasnier; A Danchin; F O'Gara
Journal: J Bacteriol Date: 1990-05 Impact factor: 3.490

3. RNA-binding activity of the matK protein encoded by the chloroplast trnK intron from mustard (Sinapis alba L.).

Authors: K Liere; G Link
Journal: Nucleic Acids Res Date: 1995-03-25 Impact factor: 16.971

4. Identification of the mcrD gene product and its association with component C of methyl coenzyme M reductase in Methanococcus vannielii.

Authors: B A Sherf; J N Reeve
Journal: J Bacteriol Date: 1990-04 Impact factor: 3.490

5. nit-2, the major positive-acting nitrogen regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein.

Authors: Y H Fu; G A Marzluf
Journal: Proc Natl Acad Sci U S A Date: 1990-07 Impact factor: 11.205

One-step purification of hybrid proteins which have beta-galactosidase activity.

1. Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2.

2. Rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases.

3. RNA-binding activity of the matK protein encoded by the chloroplast trnK intron from mustard (Sinapis alba L.).

4. Identification of the mcrD gene product and its association with component C of methyl coenzyme M reductase in Methanococcus vannielii.

5. nit-2, the major positive-acting nitrogen regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein.

6. The regulatory region of the uxuAB operon in Escherichia coli K12.

7. Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II.

8. Escherichia coli-mycobacteria shuttle vectors for operon and gene fusions to lacZ: the pJEM series.

9. Cellular responses to a 55-kilodalton recombinant Pneumocystis carinii antigen.

Review 10. Strategies for achieving high-level expression of genes in Escherichia coli.