Literature DB >> 3049659

Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II.

C Handl1, B Rönnberg, B Nilsson, E Olsson, H Jonsson, J I Flock.   

Abstract

The gene for Escherichia coli heat-stable enterotoxin type II (STII) was fused to the genes for protein A from Staphylococcus aureus and beta-galactosidase in two different expression systems. Antibodies raised in rabbits against the protein A-STII fusion protein recognized the beta-galactosidase-STII fusion protein. The latter fusion protein was used as the immobilized antigen in an enzyme-linked immunosorbent assay (ELISA) for detection of STII. The correlation between the results of the ELISA and the intestinal loop test in piglets was 95%, suggesting that the ELISA can be used to reliably detect STII.

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Year:  1988        PMID： 3049659      PMCID： PMC266659          DOI： 10.1128/jcm.26.8.1555-1560.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  33 in total

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Authors:  S Iordănescu
Journal:  J Bacteriol       Date:  1975-11       Impact factor: 3.490

2.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

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Authors:  O Söderlind
Journal:  Zentralbl Veterinarmed B       Date:  1973-09

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Authors:  D J Evans; L C Chen; G T Curlin; D G Evans
Journal:  Nat New Biol       Date:  1972-04-05

5.  Activation of intestinal guanylate cyclase by heat-stable enterotoxin of Escherichia coli: studies of tissue specificity, potential receptors, and intermediates.

Authors:  R L Guerrant; J M Hughes; B Chang; D C Robertson; F Murad
Journal:  J Infect Dis       Date:  1980-08       Impact factor: 5.226

6.  Biological evaluation of a methanol-soluble, heat-stable Escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves.

Authors:  M N Burgess; R J Bywater; C M Cowley; N A Mullan; P M Newsome
Journal:  Infect Immun       Date:  1978-08       Impact factor: 3.441

7.  Immunological properties of Escherichia coli heat-stable enterotoxins: development of a radioimmunoassay specific for heat-stable enterotoxins with suckling mouse activity.

Authors:  J C Frantz; D C Robertson
Journal:  Infect Immun       Date:  1981-07       Impact factor: 3.441

8.  Adenosine diphosphate-ribosylation of adenylate cyclase catalyzed by heat-labile enterotoxin of Escherichia coli: comparison with cholera toxin.

Authors:  D M Gill; S H Richardson
Journal:  J Infect Dis       Date:  1980-01       Impact factor: 5.226

9.  Comparison of different assays for definition of heat-stable enterotoxigenicity of Escherichia coli porcine strains.

Authors:  E Olsson; O Söderlind
Journal:  J Clin Microbiol       Date:  1980-01       Impact factor: 5.948

10.  Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.

Authors:  K Itakura; T Hirose; R Crea; A D Riggs; H L Heyneker; F Bolivar; H W Boyer
Journal:  Science       Date:  1977-12-09       Impact factor: 47.728
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  7 in total

1.  High-level production of Escherichia coli STb heat-stable enterotoxin and quantification by a direct enzyme-linked immunosorbent assay.

Authors:  R G Urban; E M Pipper; L A Dreyfus; S C Whipp
Journal:  J Clin Microbiol       Date:  1990-11       Impact factor: 5.948

2.  Assay for enterotoxigenic Escherichia coli heat-stable toxin b in rats and mice.

Authors:  S C Whipp
Journal:  Infect Immun       Date:  1990-04       Impact factor: 3.441

3.  Immunodot detection of Escherichia coli heat-stable enterotoxin b by using enhanced chemiluminescence reaction.

Authors:  L A Lortie; J Harel; J M Fairbrother; J D Dubreuil
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948

4.  Production and purification of heat-stable enterotoxin b from a porcine Escherichia coli strain.

Authors:  J D Dubreuil; J M Fairbrother; R Lallier; S Larivière
Journal:  Infect Immun       Date:  1991-01       Impact factor: 3.441

Review 5.  Swine and cattle enterotoxigenic Escherichia coli-mediated diarrhea. Development of therapies based on inhibition of bacteria-host interactions.

Authors:  M Mouricout
Journal:  Eur J Epidemiol       Date:  1991-11       Impact factor: 8.082

6.  Effects of Lactobacillus johnsonii and Lactobacillus reuteri on gut barrier function and heat shock proteins in intestinal porcine epithelial cells.

Authors:  Hao-Yu Liu; Stefan Roos; Hans Jonsson; David Ahl; Johan Dicksved; Jan Erik Lindberg; Torbjörn Lundh
Journal:  Physiol Rep       Date:  2015-04

7.  Lactobacillus reuteri strains protect epithelial barrier integrity of IPEC-J2 monolayers from the detrimental effect of enterotoxigenic Escherichia coli.

Authors:  Shokoufeh Karimi; Hans Jonsson; Torbjörn Lundh; Stefan Roos
Journal:  Physiol Rep       Date:  2018-01
  7 in total

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