Literature DB >> 6425414

Immunohistological screening in the selection of monoclonal antibodies: the use of isotype-specific antiglobulins.

N M Aqel, M Clark, S P Cobbold, H Waldmann.   

Abstract

This paper describes the use of the immunoperoxidase technique for the screening of rat hybridoma culture supernatants on tissue sections. By combining the avidin-biotin system with mouse monoclonal antibodies specific to different rat immunoglobulin isotypes, it is possible to resolve the specificity patterns of complex mixtures of monoclonal antibodies from uncloned culture wells. This strategy is particularly useful in the derivation of monoclonal antibodies to cell surface antigens.

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Year:  1984        PMID： 6425414     DOI： 10.1016/0022-1759(84)90319-3

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  5 in total

1.  A role for CD4+ but not CD8+ T cells in immunity to Schistosoma mansoni induced by 20 krad-irradiated and Ro 11-3128-terminated infections.

Authors:  D A Vignali; P Crocker; Q D Bickle; S Cobbold; H Waldmann; M G Taylor
Journal:  Immunology       Date:  1989-08       Impact factor: 7.397

2.  Pathogenic mechanisms in murine autoimmune thyroiditis: short- and long-term effects of in vivo depletion of CD4+ and CD8+ cells.

Authors:  Y M Kong; H Waldmann; S Cobbold; A A Giraldo; B E Fuller; L L Simon
Journal:  Clin Exp Immunol       Date:  1989-09       Impact factor: 4.330

3.  Histochemistry of dipeptidyl aminopeptidase (DAP) II and IV in reactive lymphoid tissues and malignant lymphoma.

Authors:  M R Khalaf; N M Aqel; F G Hayhoe
Journal:  J Clin Pathol       Date:  1987-05       Impact factor: 3.411

4.  Induction of classical transplantation tolerance in the adult.

Authors:  S X Qin; S Cobbold; R Benjamin; H Waldmann
Journal:  J Exp Med       Date:  1989-03-01       Impact factor: 14.307

5.  Tolerance to rat monoclonal antibodies. Implications for serotherapy.

Authors:  R J Benjamin; S P Cobbold; M R Clark; H Waldmann
Journal:  J Exp Med       Date:  1986-06-01       Impact factor: 14.307
  5 in total

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