Literature DB >> 6425075

Purification and properties of ornithine aminotransferase from rat brain.

D R Deshmukh, S K Srivastava.   

Abstract

Ornithine aminotransferase (E.C. 2.6.1.13) from rat brain was purified 100-fold by ammonium sulphate fractionation, DEAE cellulose chromatography, calcium phosphate gel and alumina C gamma gel. Pyridoxal phosphate was essential for maximum activity of the enzyme. The brain enzyme did not differ from liver and kidney enzymes in properties such as pH optimum, Km, substrate specificity and the inhibition by branched chain amino acids. Unlike rat liver enzyme, brain ornithine aminotransferase was able to catalyze the reaction between L-lysine and 2-oxoglutarate. Spermidine and spermine inhibited brain ornithine aminotransferase activity.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6425075     DOI： 10.1007/bf01952550

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  16 in total

1.  Studies on functional and metabolic role of urea cycle intermediates in brain.

Authors:  B Sadasivudu; T I Rao
Journal:  J Neurochem       Date:  1976-09       Impact factor: 5.372

2.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

3.  PURIFICATION AND PROPERTIES OF RAT LIVER ORNITHINE DELTA-TRANSAMINASE.

Authors:  H J STRECKER
Journal:  J Biol Chem       Date:  1965-03       Impact factor: 5.157

4.  STUDIES ON ORNITHINE KETOACID TRANSAMINASE. II. ROLE IN METABOLIC PATHWAY.

Authors:  N KATUNUMA; M OKADA; T MATSUZAWA; Y OTSUKA
Journal:  J Biochem       Date:  1965-03       Impact factor: 3.387

5.  Citrulline synthesis in rat tissues.

Authors:  M E JONES; A D ANDERSON; C ANDERSON; S HODES
Journal:  Arch Biochem Biophys       Date:  1961-12       Impact factor: 4.013

6.  Enzymatic transamination reactions involving arginine and ornithine.

Authors:  A MEISTER
Journal:  J Biol Chem       Date:  1954-02       Impact factor: 5.157

Review 7.  Two carbamyl phosphate synthetases of mammals: specific roles in control of pyrimidine and urea biosynthesis.

Authors:  M Tatibana; K Shigesada
Journal:  Adv Enzyme Regul       Date:  1972

8.  Ornithine transaminase in the liver of the chick embryo and in the young chick.

Authors:  D A Vecchio; S M Kalman
Journal:  Arch Biochem Biophys       Date:  1968-09-20       Impact factor: 4.013

9.  Purification and properties of rat kidney and liver ornithine aminotransferase.

Authors:  M C Yip; R K Collins
Journal:  Enzyme       Date:  1971

10.  A new synaptosomal biosynthetic pathway of glutamate and GABA from ornithine and its negative feedback inhibition by GABA.

Authors:  Y Yoneda; E Roberts; G W Dietz
Journal:  J Neurochem       Date:  1982-06       Impact factor: 5.372
View more
  4 in total

1.  A sensitive assay for ornithine amino transferase in rat brain mitochondria by ninhydrin method.

Authors:  H Ravi Kumar; S Ananda; K S Devaraju; B M Prakash; S Sampath Kumar; S V Suresh Babu; N Ramachandraswamy; H P Puttaraju
Journal:  Indian J Clin Biochem       Date:  2009-09-16

2.  5-Fluoromethylornithine, an irreversible and specific inhibitor of L-ornithine:2-oxo-acid aminotransferase.

Authors:  G Daune; F Gerhart; N Seiler
Journal:  Biochem J       Date:  1988-07-15       Impact factor: 3.857

3.  Molecular cloning of human ornithine aminotransferase mRNA.

Authors:  G Inana; S Totsuka; M Redmond; T Dougherty; J Nagle; T Shiono; T Ohura; E Kominami; N Katunuma
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

Review 4.  Ornithine Aminotransferase, an Important Glutamate-Metabolizing Enzyme at the Crossroads of Multiple Metabolic Pathways.

Authors:  Antonin Ginguay; Luc Cynober; Emmanuel Curis; Ioannis Nicolis
Journal:  Biology (Basel)       Date:  2017-03-07
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.