Literature DB >> 6416165

Isolation and characterization of Corynebacterium diphtheriae nontandem double lysogens hyperproducing CRM197.

R Rappuoli.   

Abstract

Phage beta 197tox-, which codes for CRM197, a nontoxic protein immunochemically indistinguishable from diphtheria toxin, was UV induced from a culture of the C7(beta 197)tox- strain. A total of 191 C7(beta 197)tox- lysogens were isolated and selected according to the halo produced on TYE agar containing antidiphtheria toxin serum and were further characterized by Southern blots of their chromosomal DNA. Most of the isolates turned out to be monolysogens, but some tandem and nontandem double lysogens were also found. The nontandem double lysogens were stable and capable of giving high yields of CRM197, up to threefold higher than monolysogens. They are, therefore, suitable for large-scale industrial production.

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Year:  1983        PMID： 6416165      PMCID： PMC239316          DOI： 10.1128/aem.46.3.560-564.1983

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

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Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

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Authors:  W L BARDSDALE; A M PAPPENHEIMER
Journal:  J Bacteriol       Date:  1954-02       Impact factor: 3.490

3.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

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Authors:  A M Pappenheimer; T Uchida; A A Harper
Journal:  Immunochemistry       Date:  1972-09

5.  Determination of Corynebacterium diphtheriae toxigenicity by a colorimetric tissue culture assay.

Authors:  J R Murphy; P Bacha; M Teng
Journal:  J Clin Microbiol       Date:  1978-01       Impact factor: 5.948

6.  Diphtheria toxin and related proteins. I. Isolation and properties of mutant proteins serologically related to diphtheria toxin.

Authors:  T Uchida; A M Pappenheimer; R Greany
Journal:  J Biol Chem       Date:  1973-06-10       Impact factor: 5.157

7.  Diphtheria toxin and related proteins. II. Kinetic studies on intoxication of HeLa cells by diphtheria toxin and related proteins.

Authors:  T Uchida; A M Pappenheimer; A A Harper
Journal:  J Biol Chem       Date:  1973-06-10       Impact factor: 5.157

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Authors:  J Blass; B Bizzini; M Raynaud
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1965-08-02

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Authors:  C Kanei; T Uchida; M Yoneda
Journal:  Infect Immun       Date:  1977-10       Impact factor: 3.441

10.  Mutation in the structural gene for diphtheria toxin carried by temperate phage .

Authors:  T Uchida; D M Gill; A M Pappenheimer
Journal:  Nat New Biol       Date:  1971-09-01
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  12 in total

1.  Subunit S1 of pertussis toxin: mapping of the regions essential for ADP-ribosyltransferase activity.

Authors:  M Pizza; A Bartoloni; A Prugnola; S Silvestri; R Rappuoli
Journal:  Proc Natl Acad Sci U S A       Date:  1988-10       Impact factor: 11.205

2.  Genome organization and pathogenicity of Corynebacterium diphtheriae C7(-) and PW8 strains.

Authors:  Masaaki Iwaki; Takako Komiya; Akihiko Yamamoto; Akiko Ishiwa; Noriyo Nagata; Yoshichika Arakawa; Motohide Takahashi
Journal:  Infect Immun       Date:  2010-06-14       Impact factor: 3.441

3.  Characterization of recombinant tetanus toxin derivatives suitable for vaccine development.

Authors:  D Figueiredo; C Turcotte; G Frankel; Y Li; O Dolly; G Wilkin; D Marriott; N Fairweather; G Dougan
Journal:  Infect Immun       Date:  1995-08       Impact factor: 3.441

4.  Physical map of the chromosomal region of Corynebacterium diphtheriae containing corynephage attachment sites attB1 and attB2.

Authors:  R Rappuoli; G Ratti
Journal:  J Bacteriol       Date:  1984-04       Impact factor: 3.490

5.  The amino-acid sequence of two non-toxic mutants of diphtheria toxin: CRM45 and CRM197.

Authors:  G Giannini; R Rappuoli; G Ratti
Journal:  Nucleic Acids Res       Date:  1984-05-25       Impact factor: 16.971

6.  Detection of homology to the beta bacteriophage integration site in a wide variety of Corynebacterium spp.

Authors:  N Cianciotto; R Rappuoli; N Groman
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

7.  Effect of increased CRM₁₉₇ carrier protein dose on meningococcal C bactericidal antibody response.

Authors:  Lucia H Lee; Milan S Blake
Journal:  Clin Vaccine Immunol       Date:  2012-02-15

8.  DNA relationships among some tox-bearing corynebacteriophages.

Authors:  G A Buck; R E Cross; T P Wong; J Loera; N Groman
Journal:  Infect Immun       Date:  1985-09       Impact factor: 3.441

9.  Construction of Bordetella pertussis strains with enhanced production of genetically-inactivated Pertussis Toxin and Pertactin by unmarked allelic exchange.

Authors:  Wasin Buasri; Attawut Impoolsup; Chuenchit Boonchird; Anocha Luengchaichawange; Pannipa Prompiboon; Jean Petre; Watanalai Panbangred
Journal:  BMC Microbiol       Date:  2012-04-23       Impact factor: 3.605

10.  The Pneumococcal Polysaccharide-Tetanus Toxin Native C-Fragment Conjugate Vaccine: The Carrier Effect and Immunogenicity.

Authors:  Rui Yu; Junjie Xu; Tao Hu; Wei Chen
Journal:  Mediators Inflamm       Date:  2020-07-04       Impact factor: 4.711
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