Literature DB >> 6413824

Construction of recombination-deficient strains of Pseudomonas aeruginosa.

R Früh, J M Watson, D Haas.   

Abstract

The rec-102 mutation had pleiotropic effects in Pseudomonas aeruginosa: low recombinational proficiency in conjugation and transduction; high UV sensitivity; inability to induce pyocin R2 by mitomycin C; and increased susceptibility to mitomycin C and nalidixic acid. The rec-102 locus was mapped by R68.45-mediated conjugation in the 45 min region of the PAO chromosome, between argF and thr-9001. By selection for a marker in this region, rec-102 can be introduced into a P. aeruginosa strain of interest using an R68.45 rec-102 donor. The recombination-deficient strains constructed in this way were phenotypically similar to Escherichia coli recA mutants.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6413824     DOI： 10.1007/BF00334835

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  19 in total

1.  A mutant of Pseudomonas aeruginosa deficient in an ATP-dependent deoxyribonuclease.

Authors:  P R Lehrbach; C D Dirckze; B T Lee
Journal:  J Gen Microbiol       Date:  1980-10

2.  Genetic circularity of the Pseudomonas aeruginosa PAO chromosome.

Authors:  P L Royle; H Matsumoto; B W Holloway
Journal:  J Bacteriol       Date:  1981-01       Impact factor: 3.490

Review 3.  The SOS regulatory system of Escherichia coli.

Authors:  J W Little; D W Mount
Journal:  Cell       Date:  1982-05       Impact factor: 41.582

4.  A novel transducing phage. Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa.

Authors:  V Krishnapillai
Journal:  Mol Gen Genet       Date:  1972

5.  Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny.

Authors:  R V Miller; C M Ku
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

6.  Role of 4-aminobutyrate aminotransferase in the arginine metabolism of Pseudomonas aeruginosa.

Authors:  R Voellym; T Leisinger
Journal:  J Bacteriol       Date:  1976-12       Impact factor: 3.490

7.  Chromosome mobilization by the R plasmid R68.45: a tool in Pseudomonas genetics.

Authors:  D Haas; B W Holloway
Journal:  Mol Gen Genet       Date:  1978-01-17

8.  The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa.

Authors:  D Haas; B W Holloway; A Schamböck; T Leisinger
Journal:  Mol Gen Genet       Date:  1977-07-07

9.  Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome.

Authors:  R H Olsen; G DeBusscher; W R McCombie
Journal:  J Bacteriol       Date:  1982-04       Impact factor: 3.490

10.  Spontaneous deletions of the chromosome-mobilizing plasmid R68.45 in Pseudomonas aeruginosa PAO.

Authors:  D Haas; G Riess
Journal:  Plasmid       Date:  1983-01       Impact factor: 3.466
View more
  19 in total

1.  The sequence and function of the recA gene and its protein in Pseudomonas aeruginosa PAO.

Authors:  Y Sano; M Kageyama
Journal:  Mol Gen Genet       Date:  1987-07

2.  Biological cost of pyocin production during the SOS response in Pseudomonas aeruginosa.

Authors:  Jon Penterman; Pradeep K Singh; Graham C Walker
Journal:  J Bacteriol       Date:  2014-07-14       Impact factor: 3.490

3.  The CAM-OCT plasmid enhances UV responses of Pseudomonas aeruginosa recA mutants.

Authors:  D L McBeth
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

4.  Specialized transduction of Pseudomonas aeruginosa PAO by bacteriophage D3.

Authors:  M M Cavenagh; R V Miller
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

5.  Conjugal transfer of R68.45 and FP5 between Pseudomonas aeruginosa strains in a freshwater environment.

Authors:  S B O'Morchoe; O Ogunseitan; G S Sayler; R V Miller
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792

6.  Role of the recA-related gene adjacent to the recA gene in Pseudomonas aeruginosa.

Authors:  Y Sano
Journal:  J Bacteriol       Date:  1993-04       Impact factor: 3.490

7.  Isolation of a recombination-deficient mutant of Rhodopseudomonas capsulata.

Authors:  F J Genthner; J D Wall
Journal:  J Bacteriol       Date:  1984-12       Impact factor: 3.490

8.  Genetic aspects of biodegradation by pseudomonads.

Authors:  D Haas
Journal:  Experientia       Date:  1983-11-15

9.  Characterization of the Pseudomonas aeruginosa recA analog and its protein product: rec-102 is a mutant allele of the P. aeruginosa PAO recA gene.

Authors:  T A Kokjohn; R V Miller
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

10.  Anaerobic regulation of transcription initiation in the arcDABC operon of Pseudomonas aeruginosa.

Authors:  M Gamper; A Zimmermann; D Haas
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.