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Literature DB >> 2823059

The sequence and function of the recA gene and its protein in Pseudomonas aeruginosa PAO.

Abstract

The recA gene of Pseudomonas aeruginosa has been isolated and its nucleotide sequence has been determined. The coding region of the recA gene has 1038 bp specifying 346 amino acids. The recA protein of P. aeruginosa showed a striking homology with that of Escherichia coli except for the carboxy-terminal region both at the nucleotide and amino acid level. The recA+-carrying plasmids restored the UV sensitivity and recombination ability of several rec mutants of P. aeruginosa. The precise location of the recA gene on the chromosome was deduced from the analysis of R' plasmids.

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Year: 1987 PMID： 2823059 DOI： 10.1007/BF00328132

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

45 in total

1. Dideoxy sequencing method using denatured plasmid templates.

Authors: M Hattori; Y Sakaki
Journal: Anal Biochem Date: 1986-02-01 Impact factor: 3.365

Review 2. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.

Authors: E M Witkin
Journal: Bacteriol Rev Date: 1976-12

3. Colicin E3 and its immunity genes.

Authors: H Masaki; T Ohta
Journal: J Mol Biol Date: 1985-03-20 Impact factor: 5.469

4. Purification and properties of an S-type pyocin, pyocin AP41.

Authors: Y Sano; M Kageyama
Journal: J Bacteriol Date: 1981-05 Impact factor: 3.490

5. Organization of the recA gene of Escherichia coli.

Authors: T Horii; T Ogawa; H Ogawa
Journal: Proc Natl Acad Sci U S A Date: 1980-01 Impact factor: 11.205

6. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.

Authors: M S Blake; K H Johnston; G J Russell-Jones; E C Gotschlich
Journal: Anal Biochem Date: 1984-01 Impact factor: 3.365

7. Cysteinyl residues of Escherichia coli recA protein.

Authors: S Kuramitsu; K Hamaguchi; H Tachibana; T Horii; T Ogawa; H Ogawa
Journal: Biochemistry Date: 1984-05-22 Impact factor: 3.162

8. Isolation and characterization of the Vibrio cholerae recA gene.

Authors: A N Hamood; G S Pettis; C D Parker; M A McIntosh
Journal: J Bacteriol Date: 1986-07 Impact factor: 3.490

9. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.

Authors: M Bagdasarian; R Lurz; B Rückert; F C Franklin; M M Bagdasarian; J Frey; K N Timmis
Journal: Gene Date: 1981-12 Impact factor: 3.688

10. Genetic determinant of pyocin AP41 as an insert in the Pseudomonas aeruginosa chromosome.

Authors: Y Sano; M Kageyama
Journal: J Bacteriol Date: 1984-05 Impact factor: 3.490

27 in total

1. Recombinogenic activity of chimeric recA genes (Pseudomonas aeruginosa/Escherichia coli): a search for RecA protein regions responsible for this activity.

Authors: I V Bakhlanova; T Ogawa; V A Lanzov
Journal: Genetics Date: 2001-09 Impact factor: 4.562

2. Expression of the recA gene of Escherichia coli in several species of gram-negative bacteria.

Authors: A R Fernandez de Henestrosa; S Calero; J Barbé
Journal: Mol Gen Genet Date: 1991-05

3. Nucleotide sequence and further characterization of the Synechococcus sp. strain PCC 7002 recA gene: complementation of a cyanobacterial recA mutation by the Escherichia coli recA gene.

Authors: R C Murphy; G E Gasparich; D A Bryant; R D Porter
Journal: J Bacteriol Date: 1990-02 Impact factor: 3.490

The sequence and function of the recA gene and its protein in Pseudomonas aeruginosa PAO.

1. Dideoxy sequencing method using denatured plasmid templates.

Review 2. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.

3. Colicin E3 and its immunity genes.

4. Purification and properties of an S-type pyocin, pyocin AP41.

5. Organization of the recA gene of Escherichia coli.

6. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.

7. Cysteinyl residues of Escherichia coli recA protein.

8. Isolation and characterization of the Vibrio cholerae recA gene.

9. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.

10. Genetic determinant of pyocin AP41 as an insert in the Pseudomonas aeruginosa chromosome.

1. Recombinogenic activity of chimeric recA genes (Pseudomonas aeruginosa/Escherichia coli): a search for RecA protein regions responsible for this activity.

2. Expression of the recA gene of Escherichia coli in several species of gram-negative bacteria.

3. Nucleotide sequence and further characterization of the Synechococcus sp. strain PCC 7002 recA gene: complementation of a cyanobacterial recA mutation by the Escherichia coli recA gene.

4. Biological cost of pyocin production during the SOS response in Pseudomonas aeruginosa.

5. The CAM-OCT plasmid enhances UV responses of Pseudomonas aeruginosa recA mutants.

6. Nucleotide sequence of the recA gene of Proteus mirabilis.

7. Expression of the recA gene of Pseudomonas aeruginosa PAO is inducible by DNA-damaging agents.

8. Cloning of the Helicobacter pylori recA gene and functional characterization of its product.

9. Regulation of the expression of the Pseudomonas stutzeri recA gene.

10. Development and characterization of recA mutants of Campylobacter jejuni for inclusion in attenuated vaccines.