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Literature DB >> 6405741

Assessment of the flux of mitochondrial acetyl-CoA in liver and kidney by using the differential production of 14CO2 from tracers of (1-14C)- and (2-14C)-labelled 4-methyl-2-oxovalerate.

Abstract

A procedure is described to convert rates of (14)CO(2) production into rates of mitochondrial acetyl-CoA production from a (14)C-labelled substrate. The principle is illustrated in perfused rat liver and kidney by the differential yield of (14)CO(2) from 4-methyl-2-oxo[1-(14)C]valerate and 4-methyl-2-oxo[2-(14)C]valerate.

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Year: 1983 PMID： 6405741 PMCID： PMC1154214 DOI： 10.1042/bj2100265

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

16 in total

1. Synthesis via the Krebs' cycle in the utilization of acetate by rat liver slices.

Authors: J KATZ; I L CHAIKOFF
Journal: Biochim Biophys Acta Date: 1955-09

2. Transfer of carbon atoms from mevalonate to n-fatty acids.

Authors: J Edmond; G Popják
Journal: J Biol Chem Date: 1974-01-10 Impact factor: 5.157

3. Fatty acid and 3- -hydroxysterol synthesis in the perfused rat liver. Including measurements on the production of lactate, pyruvate, -hydroxy-butyrate, and acetoacetate by the fed liver.

Authors: H Brunengraber; M Boutry; J M Lowenstein
Journal: J Biol Chem Date: 1973-04-25 Impact factor: 5.157

4. A simplified method for the preparation of 14 C-labelled branched-chain -oxo acids.

Authors: H W Rüdiger; U Langenbeck; H W Goedde
Journal: Biochem J Date: 1972-01 Impact factor: 3.857

5. Citrate and the conversion of carbohydrate into fat. Fatty acid synthesis by a combination of cytoplasm and mitochondria.

Authors: J A Watson; J M Lowenstein
Journal: J Biol Chem Date: 1970-11-25 Impact factor: 5.157

6. Subcellular distribution and partial characterization of an alpha-ketoisocaproate oxidase of rat liver: formation of beta-hydroxyisovaleric acid.

Authors: P J Sabourin; L L Bieber
Journal: Arch Biochem Biophys Date: 1981-01 Impact factor: 4.013

Assessment of the flux of mitochondrial acetyl-CoA in liver and kidney by using the differential production of 14CO2 from tracers of (1-14C)- and (2-14C)-labelled 4-methyl-2-oxovalerate.

1. Synthesis via the Krebs' cycle in the utilization of acetate by rat liver slices.

2. Transfer of carbon atoms from mevalonate to n-fatty acids.

3. Fatty acid and 3- -hydroxysterol synthesis in the perfused rat liver. Including measurements on the production of lactate, pyruvate, -hydroxy-butyrate, and acetoacetate by the fed liver.

4. A simplified method for the preparation of 14 C-labelled branched-chain -oxo acids.

5. Citrate and the conversion of carbohydrate into fat. Fatty acid synthesis by a combination of cytoplasm and mitochondria.

6. Subcellular distribution and partial characterization of an alpha-ketoisocaproate oxidase of rat liver: formation of beta-hydroxyisovaleric acid.

7. The fate of isotopic carbon in kidney cortex synthesizing glucose from lactate.

8. Studies on the regulation of the branched chain alpha-keto acid dehydrogenase in the perfused rat liver.

9. Leucine degradation in cell-free extracts of skeletal muscle.

10. Regulation of alpha-ketoisocaproate binding to albumin in vivo by free fatty acids.

1. The stimulation of tricarboxylic acid-cycle flux by alpha-adrenergic agonists in perfused rat liver.