Literature DB >> 6405659

Accurate assay of dopa decarboxylase by preventing nonenzymatic decarboxylation of dopa.

S Okuno, H Fujisawa.   

Abstract

The nonenzymatic decarboxylation of dopa was completely blocked by both 2-mercaptoethanol and EDTA together over the wide range of pH. This finding made it possible to measure the activity of dopa decarboxylase precisely even at an alkaline pH value. The pH optimum of dopa decarboxylase was found to be pH 7.0 and the Km value for dopa was determined to be 4 X 10(-5) M.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6405659     DOI： 10.1016/0003-2697(83)90570-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

Review 1.  Approaches to the design of biochemical probes for positron emission tomography.

Authors:  J R Barrio
Journal:  Neurochem Res       Date:  1991-09       Impact factor: 3.996

2.  Delimiting regulatory sequences of the Drosophila melanogaster Ddc gene.

Authors:  J Hirsh; B A Morgan; S B Scholnick
Journal:  Mol Cell Biol       Date:  1986-12       Impact factor: 4.272

3.  The effects of monoamine oxidase B inhibition on dopamine metabolism in rats with nigro-striatal lesions.

Authors:  E Scarr; D M Wingerchuk; A V Juorio; I A Paterson
Journal:  Neurochem Res       Date:  1994-02       Impact factor: 3.996

4.  Regulation of aromatic L-amino acid decarboxylase in rat striatal synaptosomes: effects of dopamine receptor agonists and antagonists.

Authors:  M Y Zhu; A V Juorio; I A Paterson; A A Boulton
Journal:  Br J Pharmacol       Date:  1994-05       Impact factor: 8.739

5.  The Drosophila virilis dopa decarboxylase gene is developmentally regulated when integrated into Drosophila melanogaster.

Authors:  S J Bray; J Hirsh
Journal:  EMBO J       Date:  1986-09       Impact factor: 11.598

6.  A high molecular weight non-bombesin/gastrin releasing peptide growth factor in small cell lung cancer.

Authors:  V Macaulay; G P Joshi; M Everard; I E Smith; J L Millar
Journal:  Br J Cancer       Date:  1987-12       Impact factor: 7.640
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.