| Literature DB >> 6404566 |
N W Tietz, C A Burtis, P Duncan, K Ervin, C J Petitclerc, A D Rinker, D Shuey, E R Zygowicz.
Abstract
We present an official AACC reference method for the measurement of alkaline phosphatase, the culmination of optimization experiments conducted by a group of independent laboratories. The details of this method and evaluation of factors affecting the measurement are described. A metal ion buffer has been incorporated that maintains optimal and constant concentrations of zinc(II) and magnesium(II) ions. Final reaction conditions are: pH (30 degrees C), 10.40 +/- 0.05; 2-amino-2-methyl-1-propanol buffer, 0.35 mol/L; 4-nitrophenyl phosphate, 16.0 mmol/L; magnesium acetate, 2.0 mmol/L; zinc sulfate, 1.0 mmol/L; and N-(2-hydroxyethyl)ethylenediaminetriacetic acid, 2.0 mmol/L.Entities:
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Year: 1983 PMID： 6404566
Source DB: PubMed Journal: Clin Chem ISSN： 0009-9147 Impact factor: 8.327