Literature DB >> 6398132

Field evaluation of ELISA using Wuchereria bancrofti mf ES antigen for bancroftian filariasis.

B C Harinath, A Malhotra, S N Ghirnikar, S D Annadate, V P Isaacs, M S Bharti.   

Abstract

An enzyme-linked immunosorbent assay using Wuchereria bancrofti microfilarial excretory-secretory antigen was used in field studies to screen blood samples collected on filter-paper from persons residing in areas endemic for bancroftian filariasis. This assay system, when compared with examination of night wet blood smears for microfilariae, gave a relative sensitivity of 98% and a relative specificity of 86%. Daytime blood samples can also be used in this test, which can thus replace tedious examination of night blood samples in field surveys in endemic areas.

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Year:  1984        PMID： 6398132      PMCID： PMC2536260     

Source DB:  PubMed          Journal:  Bull World Health Organ        ISSN： 0042-9686            Impact factor:   9.408


  9 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Hyperendemic bancroftian filariasis in the Kingdom of Tonga: the application of the membrane filter concentration technique to an age-stratified blood survey.

Authors:  R S Desowitz; J C Hitchcock
Journal:  Am J Trop Med Hyg       Date:  1974-09       Impact factor: 2.345

3.  Studies of filariasis in the Pacific. 1. A field trial of a counting-chamber technique for the determination of microfilarial rates and densities.

Authors:  B A Southgate
Journal:  Southeast Asian J Trop Med Public Health       Date:  1973-06       Impact factor: 0.267

4.  Comparison of a counting chamber and thick smear methods of counting microfilariae.

Authors:  D A Denham; D T Dennis; T Ponnudurai; G S Nelson; F Guy
Journal:  Trans R Soc Trop Med Hyg       Date:  1971       Impact factor: 2.184

5.  Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

Authors:  S Avrameas
Journal:  Immunochemistry       Date:  1969-01

6.  [Evaluation of 2 immunological tests (skin test and complement fixation test) for the detection of filariasis in populations of Upper Volta where Wuchereria bancrofti, Onchocercă volvulus and Dipetalonema perstans occur together].

Authors:  R Gidel; J Brengues; F Rodhain
Journal:  Bull World Health Organ       Date:  1969       Impact factor: 9.408

7.  Present status of the filariasis problem in India.

Authors:  S P Sharma; H Biswas; M Das; S R Dwivedi
Journal:  J Commun Dis       Date:  1983-03

8.  Antibody analysis in human filarial sera by ELISA using Wuchereria bancrofti microfilariae culture antigen.

Authors:  I Kharat; B C Harinath; S N Ghirnikar
Journal:  Indian J Exp Biol       Date:  1982-05       Impact factor: 0.818

9.  Hybridoma antibody-based competitive ELISA in Schistosoma japonicum infection.

Authors:  G F Mitchell; R R Premier; E G Garcia; J G Hurrell; H M Chandler; K M Cruise; F P Tapales; W U Tiu
Journal:  Am J Trop Med Hyg       Date:  1983-01       Impact factor: 2.345
  9 in total
  3 in total

1.  Congenital filarial lymphoedema.

Authors:  P Chaturvedi; B Advani; A A Khan; B C Harinath; A Gawdi
Journal:  Indian J Pediatr       Date:  1991 May-Jun       Impact factor: 1.967

2.  Immunomonitoring followed by optimal dec therapy for successful management of clinical filariasis in an endemic area.

Authors:  B C Harinath; M V Reddy; R Alli; V K Mehta; P Chaturvedi; K R Patond; S P Kalantri; R K Gupta
Journal:  Indian J Clin Biochem       Date:  1999-07

3.  Filaria associated clinical manifestations in children in an endemic area and morbidity control by immunomonitoring and optimal DEC therapy: Sevagram experience.

Authors:  B C Harinath; M V Reddy; B Bhunia; Y P Bhandari; V K Mehta; P Chaturvedi; N C Prajapati; R K Gupta
Journal:  Indian J Clin Biochem       Date:  2000-08
  3 in total

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