Literature DB >> 6392888

Enzyme-linked immunosorbent assay (ELISA) in the paracoccidioidomycosis. Comparison with counterimmunoelectrophoresis and erythro-immunoassay.

Z P de Camargo, J L Guesdon, E Drouhet, L Improvisi.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) for detection and quantification of antibodies anti-Paracoccidioides brasiliensis is described. Polystyrene plates have been used as solid phase to absorb P. brasiliensis metabolic yeast phase antigen. Twenty sera of proven paracoccidioidomycosis, 11 of histoplasmosis due Histoplasma capsulatum, 20 of aspergillosis and 20 human normal sera were tested. Ninety-five percent of the paracoccidioidomycosis sera had O.D. superior to 0.150 (from 0.163 to 2.650) at 1/400 serum dilution. ELISA assay was compared with counterimmunoelectrophoresis and erythro-immunoassay tests; a correlation was observed only with erythro-immunoassay. ELISA test should give new perspectives for the serodiagnosis of paracoccidioidomycosis.

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Year:  1984        PMID: 6392888     DOI: 10.1007/BF00439292

Source DB:  PubMed          Journal:  Mycopathologia        ISSN: 0301-486X            Impact factor:   2.574


  32 in total

1.  [Evaluation of a specific double-diffusion technique in paracoccidioidomycosis diagnosis (author's transl)].

Authors:  L A Yarzábal; N A de Cabral; A R Santiago
Journal:  Acta Cient Venez       Date:  1979

2.  Enzyme linked immunosorbent assay (ELISA) for IgG and IgE antibodies to protein and polysaccharide antigens of Aspergillus fumigatus.

Authors:  R Sepulveda; J L Longbottom; J Pepys
Journal:  Clin Allergy       Date:  1979-07

3.  Isolation of a specific antigen with alkaline phosphatase activity from soluble extracts of Paracoccidioides brasiliensis.

Authors:  L A Yarzabal; S Andrieu; D Bout; F Naquira
Journal:  Sabouraudia       Date:  1976-11

4.  [Comparative study of immunoelectrophoresis (IEF) and immunoelectroosmophoresis-immunodiffusion (IEOF-ID) used in the diagnosis of paracoccidioidomycosis].

Authors:  I A Conti-Diaz; J E Mackinnon; L Calegari; S Casserone
Journal:  Mycopathologia       Date:  1978-08-10       Impact factor: 2.574

5.  [Specific precipitant antibodies in South American blastomycosis revealed by immunoelectrophoresis].

Authors:  L A Yarzabal
Journal:  Rev Inst Med Trop Sao Paulo       Date:  1971 Sep-Oct       Impact factor: 1.846

6.  The immunology of South-American blastomycosis.

Authors:  C F Netto
Journal:  Mycopathol Mycol Appl       Date:  1965-09-30

7.  Titration of antibodies to Paracoccidioides brasiliensis by erythro-immunoassay (EIA).

Authors:  Z P de Camargo; J L Guesdon; E Drouhet; L Improvisi
Journal:  Sabouraudia       Date:  1984

8.  Enzyme-linked immunosorbent assay (ELISA) for IgM, IgG and IgA class antibodies against Candida albicans antigens: development and comparison with other methods.

Authors:  A A Kostiala; I Kostiala
Journal:  Sabouraudia       Date:  1981-06

9.  Application of enzyme-linked immunosorbent assay (ELISA) in diagnosis of allergic bronchopulmonary aspergillosis.

Authors:  P A Greenberger; R Patterson
Journal:  J Lab Clin Med       Date:  1982-02

10.  Comparison by ELISA of serum anti-Candida albicans mannan IgG levels of a normal population and in diseased patients.

Authors:  P F Lehmann; E Reiss
Journal:  Mycopathologia       Date:  1980-03-17       Impact factor: 2.574

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  22 in total

1.  Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis.

Authors:  C F Albuquerque; S H Marques da Silva; Z P Camargo
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

2.  43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplasmosis, or Jorge Lobo's disease.

Authors:  R Puccia; L R Travassos
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

Review 3.  Serology of paracoccidioidomycosis.

Authors:  Zoilo Pires de Camargo
Journal:  Mycopathologia       Date:  2008 Apr-May       Impact factor: 2.574

4.  Use of monoclonal antibodies in diagnosis of paracoccidioidomycosis: new strategies for detection of circulating antigens.

Authors:  B L Gómez; J I Figueroa; A J Hamilton; B Ortiz; M A Robledo; R J Hay; A Restrepo
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

5.  Discovery of Fluorescence Polarization Probe for the ELISA-Based Antagonist Screening of α1-Adrenergic Receptors.

Authors:  Zhao Ma; Zhenzhen Liu; Tianyu Jiang; Tianchao Zhang; Huateng Zhang; Lupei Du; Minyong Li
Journal:  ACS Med Chem Lett       Date:  2016-08-26       Impact factor: 4.345

6.  Paracoccidioides brasiliensis 87-kilodalton antigen, a heat shock protein useful in diagnosis: characterization, purification, and detection in biopsy material via immunohistochemistry.

Authors:  Soraya Díez; Beatriz L Gómez; Angela Restrepo; Rod J Hay; Andrew J Hamilton
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

7.  Combined use of Paracoccidioides brasiliensis recombinant 27-kilodalton and purified 87-kilodalton antigens in an enzyme-linked immunosorbent assay for serodiagnosis of paracoccidioidomycosis.

Authors:  Soraya Díez; Beatriz L Gómez; Juan G McEwen; Angela Restrepo; Rod J Hay; Andrew J Hamilton
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

8.  Preparation of species-specific murine monoclonal antibodies against the yeast phase of Paracoccidioides brasiliensis.

Authors:  J I Figueroa; A J Hamilton; M A Bartholomew; T Harada; L Fenelon; R J Hay
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

9.  Diagnosis of paracoccidioidomycosis by dot immunobinding assay for antibody detection using the purified and specific antigen gp43.

Authors:  C P Taborda; Z P Camargo
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948

Review 10.  Paracoccidioidomycosis: an update.

Authors:  E Brummer; E Castaneda; A Restrepo
Journal:  Clin Microbiol Rev       Date:  1993-04       Impact factor: 26.132

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