Literature DB >> 7038011

Application of enzyme-linked immunosorbent assay (ELISA) in diagnosis of allergic bronchopulmonary aspergillosis.

P A Greenberger, R Patterson.   

Abstract

IgE and IgG antibodies against Af were measured by ELISA in sera from 15 patients with ABPA and compared with antibody levels in six patients with extrinsic asthma and immediate-type skin reactivity to Af (prick skin test 3+ or 4+) but no other evidence for ABPA. The assay used polyvinyl microhemagglutination plates as a solid phase to adsorb antigens of Af and a double-antibody system of class-specific anti-human globulin and alkaline phosphatase-conjugated goat anti-rabbit globulin. IgE-Af and IgG-Af were significantly greater among patients with ABPA than in patients with asthma, suggesting that this assay may be used as an important diagnostic aid. The advantages of ELISA over radioimmunoassay are discussed in regard to early detection of an indolent pulmonary disease.

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Year:  1982        PMID： 7038011

Source DB:  PubMed          Journal:  J Lab Clin Med        ISSN： 0022-2143


  19 in total

1.  Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis.

Authors:  C F Albuquerque; S H Marques da Silva; Z P Camargo
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

2.  Cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in allergic bronchopulmonary aspergillosis.

Authors:  P W Miller; A Hamosh; M Macek; P A Greenberger; J MacLean; S M Walden; R G Slavin; G R Cutting
Journal:  Am J Hum Genet       Date:  1996-07       Impact factor: 11.025

Review 3.  Allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.

Authors:  A P Knutsen; R G Slavin
Journal:  Clin Rev Allergy       Date:  1991 Spring-Summer

Review 4.  Utility of serologic testing in the diagnosis of noninfectious pulmonary disorders.

Authors:  R H White; J A Golden
Journal:  Clin Rev Allergy       Date:  1990 Summer-Fall

5.  Detection of IgG and IgE antibodies to Aspergillus fumigatus in human sera by immunogold assay.

Authors:  H C Gugnani; K E Reijula; V P Kurup; J N Fink
Journal:  Mycopathologia       Date:  1990-01       Impact factor: 2.574

Review 6.  In vitro assays for immunoglobulin E. Methodology, indications, and interpretation.

Authors:  S G Johansson; L Yman
Journal:  Clin Rev Allergy       Date:  1988

Review 7.  Serological techniques for diagnosis of fungal infection.

Authors:  L de Repentigny
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1989-04       Impact factor: 3.267

8.  Aspergillus fumigatus-specific antibodies in allergic bronchopulmonary aspergillosis and aspergilloma: evidence for a polyclonal antibody response.

Authors:  W Brummund; A Resnick; J N Fink; V P Kurup
Journal:  J Clin Microbiol       Date:  1987-01       Impact factor: 5.948

Review 9.  New developments in the diagnosis of opportunistic fungal infection.

Authors:  V Hopwood; D W Warnock
Journal:  Eur J Clin Microbiol       Date:  1986-08       Impact factor: 3.267

10.  Precipitins and specific IgG antibody to Aspergillus fumigatus in a chest unit population.

Authors:  J A Faux; D J Shale; D J Lane
Journal:  Thorax       Date:  1992-01       Impact factor: 9.139
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