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Literature DB >> 6386584

Stabilisation of dissolved proteins against denaturation at hydrophobic interfaces.

Abstract

Studies with insulin delivery devices have shown that denaturation of dissolved proteins at hydrophobic interfaces is a basic obstacle to long-term insulin stability in pumps. This study shows that polypropylene glycol/polyethylene glycol block polymers prevent both the adsorption of dissolved proteins to hydrophobic interfaces and the resultant aggregation. At a concentration of 0.001% (w/v), the block polymer Genapol PF-10 stabilises insulin solutions over a wide range of concentrations. Insulin solutions thus stabilised are at present being clinically tested. The effectiveness of molecular variants of Genapol PF-10 to stabilise other proteins (human gamma-globulin, myoglobin and serum albumin) is presented also.

Entities: Chemical Gene Species

Mesh：

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Year: 1984 PMID： 6386584 DOI： 10.1007/bf00273809

Source DB: PubMed Journal: Diabetologia ISSN： 0012-186X Impact factor: 10.122

22 in total

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Authors: R Obermeier; R Geiger
Journal: Hoppe Seylers Z Physiol Chem Date: 1976-06

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Authors: F Macritchie
Journal: Adv Protein Chem Date: 1978

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Authors: T Kolobow; E W Stool; P K Weathersby; J Pierce; F Hayano; J Suaudeau
Journal: Trans Am Soc Artif Intern Organs Date: 1974

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Authors: C Tanford
Journal: Adv Protein Chem Date: 1970

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Authors: C Tanford
Journal: Adv Protein Chem Date: 1968

6. Some reactions of insulin at non-polar surfaces.

Authors: M Browne; R Cecil; J C Miller
Journal: Eur J Biochem Date: 1973-03-01

7. Hydrophobic free energy, micelle formation and the association of proteins with amphiphiles.

Authors: C Tanford
Journal: J Mol Biol Date: 1972-06-14 Impact factor: 5.469

Review 8. Insulin aggregation in artificial delivery systems.

Authors: W D Lougheed; H Woulfe-Flanagan; J R Clement; A M Albisser
Journal: Diabetologia Date: 1980-07 Impact factor: 10.122

9. Long-term continuous intraperitoneal insulin infusion with an implanted remote-controlled insulin infusion device.

Authors: K Irsigler; H Kritz; G Hagmüller; M Franetzki; K Prestele; H Thurow; K Geisen
Journal: Diabetes Date: 1981-12 Impact factor: 9.461

10. Prevention of insulin aggregation by dicarboxylic amino acids during prolonged infusion.

Authors: J Bringer; A Heldt; G M Grodsky
Journal: Diabetes Date: 1981-01 Impact factor: 9.461

15 in total

1. Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy.

Authors: Jesper Donsmark; Lene Jorgensen; Susanne Mollmann; Sven Frokjaer; Christian Rischel
Journal: Pharm Res Date: 2006-11-30 Impact factor: 4.200

Stabilisation of dissolved proteins against denaturation at hydrophobic interfaces.

1. A new semisynthesis of human insulin.

Review 2. Proteins at interfaces.

3. Superior blood compatibility of silicone rubber free of silica filler in the membrane lung.

Review 4. Protein denaturation. C. Theoretical models for the mechanism of denaturation.

Review 5. Protein denaturation.

6. Some reactions of insulin at non-polar surfaces.

7. Hydrophobic free energy, micelle formation and the association of proteins with amphiphiles.

Review 8. Insulin aggregation in artificial delivery systems.

9. Long-term continuous intraperitoneal insulin infusion with an implanted remote-controlled insulin infusion device.

10. Prevention of insulin aggregation by dicarboxylic amino acids during prolonged infusion.

1. Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy.

2. Displacement of adsorbed insulin by Tween 80 monitored using total internal reflection fluorescence and ellipsometry.

3. Inhibition of insulin fibrillogenesis with targeted peptides.

4. Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces.

5. Synergistic effects of surfactants and sugars on lipoplex stability during freeze-drying and rehydration.

6. In situ study of insulin aggregation induced by water-organic solvent interface.

Review 7. Pharmacokinetics of insulin. Implications for continuous subcutaneous insulin infusion therapy.

8. Chemical stability of insulin in a delivery system environment.

9. Biophysical optimization of a therapeutic protein by nonstandard mutagenesis: studies of an iodo-insulin derivative.

10. Hydrophilic microspheres from water-in-oil emulsions by the water diffusion technique.