Literature DB >> 638017

Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry.

J V Watson, P Workman, S H Chambers.   

Abstract

The reaction rates of enzymes hydrolysing fluorescein diacetate have been studied in populations of intact tissue-culture EMT6, cells using flow cytofluorimetric techniques. It was found that the activity of these enzymes increased in plateau phases and that this correlated inversely with plating efficiency. Highly abnormal substrate-dependent reaction velocity kinetics were found in 14-, 21-, 28- and 35-day cultures.

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Year:  1978        PMID： 638017      PMCID： PMC2009517          DOI： 10.1038/bjc.1978.59

Source DB:  PubMed          Journal:  Br J Cancer        ISSN： 0007-0920            Impact factor:   7.640


  11 in total

1.  Changes in cell proliferation kinetics occurring during the life history of monolayer cultures of a mouse tumour cell line.

Authors:  P R Twentyman; J V Watson; N M Bleehen; P M Rowles
Journal:  Cell Tissue Kinet       Date:  1975-01

2.  Isolation and properties of pig liver esterase.

Authors:  A J ADLER; G B KISTIAKOWSKY
Journal:  J Biol Chem       Date:  1961-12       Impact factor: 5.157

3.  A flow cytofluorimetric method for measuring enzyme reaction kinetics in intact cells.

Authors:  J V Watson; S H Chambers; P Workman; T S Hornsnell
Journal:  FEBS Lett       Date:  1977-09-01       Impact factor: 4.124

4.  Relationship between changes in the structuredness of cytoplasm and rate constants for the hydrolysis of FDA in Saccharomyces cerevisiae.

Authors:  L Cercek; B Cercek
Journal:  Biophysik       Date:  1973

5.  Structuredness of the cytoplasmic matrix and Michaelis-Menten constants for the hydrolysis of FDA during the cell cycle in Chinese hamster ovary cells.

Authors:  L Cercek; B Cercek; C H Ockey
Journal:  Biophysik       Date:  1973

6.  Characteristics of a serially transplanted mouse mammary tumor and its tissue-culture-adapted derivative.

Authors:  S C Rockwell; R F Kallman; L F Fajardo
Journal:  J Natl Cancer Inst       Date:  1972-09       Impact factor: 13.506

7.  The application of age distribution theory in the analysis of cytofluorimetric DNA histogram data.

Authors:  J V Watson
Journal:  Cell Tissue Kinet       Date:  1977-03

8.  Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

Authors:  A Krishan
Journal:  J Cell Biol       Date:  1975-07       Impact factor: 10.539

9.  Fluorescence discrimination between diploid cells on their RNA content: a possible distinction between clonogenic and non-clonogenic cells.

Authors:  J V Watson; S H Chambers
Journal:  Br J Cancer       Date:  1977-11       Impact factor: 7.640

10.  Separation of clonogenic tumour cells from EMT6 mouse mammary tumours.

Authors:  P R Twentyman; J V Watson
Journal:  Br J Cancer       Date:  1977-01       Impact factor: 7.640
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  1 in total

1.  Synthesis and Biological Evaluation of Iodoglucoazomycin (I-GAZ), an Azomycin-Glucose Adduct with Putative Applications in Diagnostic Imaging and Radiotherapy of Hypoxic Tumors.

Authors:  Piyush Kumar; Hassan R H Elsaidi; Bohdarianna Zorniak; Evelyn Laurens; Jennifer Yang; Veena Bacchu; Monica Wang; Leonard I Wiebe
Journal:  ChemMedChem       Date:  2016-07-05       Impact factor: 3.466
  1 in total

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