An evaluation of the efficiency of enrichment media in the isolation process for Vibrio parahaemolyticus.

An evaluation of the efficiency of seven enrichment media for Vibrio parahaemolyticus was effected, by means of material collected from the corporeal surface of 82 marine fish caught off the Brazilian coast. On the whole, 45 fish proved positive, from which 71 strains were taken. Among the culture media analyzed the best results were obtained with the new enrichment media represented by two of the nutrient broth (Difco) pH 8.3; one supplemented with 2 g % of NaCl and 500 mg % eosin Y, and another supplemented with 125 mg % metachrome yellow II RD. The combination of enrichment media plus selective-indicator media demonstrated the superiority of the binary association of alcaline broth eosin Y to TCBS agar, both in the number of isolations and serotypes detected.