Literature DB >> 6377304

Cross-linking of tRNA at two different sites of the elongation factor Tu.

J M Van Noort, B Kraal, L Bosch, T F La Cour, J Nyborg, B F Clark.   

Abstract

Recently, we reported on the induction by kirromycin of two tRNA binding sites on elongation factor Tu. To obtain independent information on the existence of these two sites and to characterize them further, 3' oxidized tRNA was cross-linked to elongation factor Tu by [3H]borohydride reduction. Specific cross-linking occurred exclusively in the presence of kirromycin. In the case of elongation factor Tu X GDP X kirromycin, cross-linking was found at lysine-208; in elongation factor Tu X GTP X kirromycin, cross-linking was at lysine-208 and lysine-237. In both elongation factor Tu complexes, kirromycin itself was found cross-linked to lysine-357. The tRNA cross-linking sites are in agreement with the idea of two different binding sites of tRNA on elongation factor Tu.

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Year:  1984        PMID： 6377304      PMCID： PMC345349          DOI： 10.1073/pnas.81.13.3969

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  Modification of the 3' terminus of tRNA by periodate oxidation and subsequent reaction with hydrazides.

Authors:  F Hansske; F Cramer
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

Review 2.  The role of guanosine 5'-triphosphate in polypeptide chain elongation.

Authors:  Y Kaziro
Journal:  Biochim Biophys Acta       Date:  1978-09-21

3.  High resolution x-ray crystallographic analysis of a modified form of the elongation factor Tu: guanosine diphosphate complex.

Authors:  K Morikawa; T F la Cour; J Nyborg; K M Rasmussen; D L Miller; B F Clark
Journal:  J Mol Biol       Date:  1978-11-05       Impact factor: 5.469

4.  Mechanism of the inhibition of protein synthesis by kirromycin. Role of elongation factor Tu and ribosomes.

Authors:  H Wolf; G Chinali; A Parmeggiani
Journal:  Eur J Biochem       Date:  1977-05-02

5.  Reductive alkylation of amino groups in proteins.

Authors:  G E Means; R E Feeney
Journal:  Biochemistry       Date:  1968-06       Impact factor: 3.162

6.  A simplified procedure for the isolation of bacterial polypeptide elongation factor EF-Tu.

Authors:  R Leberman; B Antonsson; R Giovanelli; R Guariguata; R Schumann; A Wittinghofer
Journal:  Anal Biochem       Date:  1980-05-01       Impact factor: 3.365

7.  Interaction of Escherichia coli EF-Tu.GTP and EF-Tu.GDP with analogues of the 3' terminus of aminoacyl-tRNA.

Authors:  J Jonák; J Smrt; A Holý; I Rychlík
Journal:  Eur J Biochem       Date:  1980-04

8.  Primary structure of human erythrocyte glycophorin A. Isolation and characterization of peptides and complete amino acid sequence.

Authors:  M Tomita; H Furthmayr; V T Marchesi
Journal:  Biochemistry       Date:  1978-10-31       Impact factor: 3.162

9.  Kirromycin, an inhibitor of protein biosynthesis that acts on elongation factor Tu.

Authors:  H Wolf; G Chinali; A Parmeggiani
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205

10.  The primary structure of the coat protein of alfalfa mosaic virus strain VRU. A hypothesis on the occurrence of two conformations in the assembly of the protein shell.

Authors:  A Castel; B Kraal; J M De Graaf; L Bosch
Journal:  Eur J Biochem       Date:  1979-12
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  10 in total

1.  Complete nucleotide sequences of seven eubacterial genes coding for the elongation factor Tu: functional, structural and phylogenetic evaluations.

Authors:  W Ludwig; M Weizenegger; D Betzl; E Leidel; T Lenz; A Ludvigsen; D Möllenhoff; P Wenzig; K H Schleifer
Journal:  Arch Microbiol       Date:  1990       Impact factor: 2.552

Review 2.  Throwing a spanner in the works: antibiotics and the translation apparatus.

Authors:  C M Spahn; C D Prescott
Journal:  J Mol Med (Berl)       Date:  1996-08       Impact factor: 4.599

3.  GTPase center of elongation factor Tu is activated by occupation of the second tRNA binding site.

Authors:  J M Van Noort; B Kraal; L Bosch
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

4.  A single amino acid substitution in elongation factor Tu disrupts interaction between the ternary complex and the ribosome.

Authors:  I Tubulekas; D Hughes
Journal:  J Bacteriol       Date:  1993-01       Impact factor: 3.490

5.  Special peptidyl-tRNA molecules can promote translational frameshifting without slippage.

Authors:  A Vimaladithan; P J Farabaugh
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

6.  A second tRNA binding site on elongation factor Tu is induced while the factor is bound to the ribosome.

Authors:  J M Van Noort; B Kraal; L Bosch
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

7.  Expression of a gene for mouse eucaryotic elongation factor Tu during murine erythroleukemic cell differentiation.

Authors:  W W Roth; P W Bragg; M V Corrias; N S Reddy; J N Dholakia; A J Wahba
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

8.  The structural and functional basis for the kirromycin resistance of mutant EF-Tu species in Escherichia coli.

Authors:  J R Mesters; L A Zeef; R Hilgenfeld; J M de Graaf; B Kraal; L Bosch
Journal:  EMBO J       Date:  1994-10-17       Impact factor: 11.598

9.  Structure-function relationships in the GTP binding domain of EF-Tu: mutation of Val20, the residue homologous to position 12 in p21.

Authors:  E Jacquet; A Parmeggiani
Journal:  EMBO J       Date:  1988-09       Impact factor: 11.598

10.  Mutants of the elongation factor EF-Tu, a new class of nonsense suppressors.

Authors:  E Vijgenboom; T Vink; B Kraal; L Bosch
Journal:  EMBO J       Date:  1985-04       Impact factor: 11.598
  10 in total

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