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Literature DB >> 6377043

Advantages and limitations of methods for measuring cellular chemotaxis and chemokinesis.

Abstract

Cell migration is an important component of many physiological reactions, for example inflammation, wound healing and formation of atherosclerotic lesions. This review summarizes the methods currently available for examining cell movement in vitro. The applications of each method, and its particular advantages and disadvantages are discussed. Techniques are described for preparing purified leukocyte populations for use in migration studies and for differentiating between chemotactic and chemokinetic cell migration.

Entities: Disease

Mesh：

Substances：
Sepharose

Year: 1984 PMID： 6377043 DOI： 10.1007/bf00230072

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

29 in total

1. Chemotaxis of granulocytes.

Authors: H HARRIS
Journal: J Pathol Bacteriol Date: 1953-07

2. Chemotaxis in leukocytes.

Authors: M McCUTCHEON
Journal: Physiol Rev Date: 1946-07 Impact factor: 37.312

3. Technetium-99m labeling of human monocytes for chemotactic studies.

Authors: C K Papierniak; R E Bourey; R R Kretschmer; S P Gotoff; L G Colombetti
Journal: J Nucl Med Date: 1976-11 Impact factor: 10.057

4. A modified method for chemotaxis under agarose.

Authors: D E Chenoweth; J G Rowe; T E Hugli
Journal: J Immunol Methods Date: 1979 Impact factor: 2.303

5. A simple multiple chamber apparatus for measuring chemotaxis of polymorphonuclear leukocytes utilizing centrifugation of the chambers before incubation.

Authors: M J Swanson
Journal: J Immunol Methods Date: 1977 Impact factor: 2.303

6. Effects of cytochalasin B on polymorphonuclear leucocyte locomotion, phagocytosis and glycolysis.

Authors: S H Zigmond; J G Hirsch
Journal: Exp Cell Res Date: 1972-08 Impact factor: 3.905

7. Re-assessment of Boyden's technique for measuring chemotaxis.

Authors: H U Keller; J F Borel; P C Wilkinson; M W Hess; H Cottier
Journal: J Immunol Methods Date: 1972-01 Impact factor: 2.303

8. Differences in superoxide production by nonmigrating and migrating human monocyte subpopulations.

Authors: L Harvath; J K Lazdins; E Alteri; E J Leonard
Journal: Biochem Biophys Res Commun Date: 1982-09-16 Impact factor: 3.575

9. Quantitative comparisons of various biological responses of neutrophils to different active and inactive chemotactic factors.

Authors: D L Kreutzer; J T O'Flaherty; W Orr; H J Showell; P A Ward; E L Becker
Journal: Immunopharmacology Date: 1978-12

10. Leukocyte migration agarose test for the assessment of human neutrophil chemotaxis. II. Variables in the attraction assay.

Authors: H Repo; T U Kosunen
Journal: Scand J Immunol Date: 1977 Impact factor: 3.487

5 in total

Advantages and limitations of methods for measuring cellular chemotaxis and chemokinesis.

1. Chemotaxis of granulocytes.

2. Chemotaxis in leukocytes.

3. Technetium-99m labeling of human monocytes for chemotactic studies.

4. A modified method for chemotaxis under agarose.

5. A simple multiple chamber apparatus for measuring chemotaxis of polymorphonuclear leukocytes utilizing centrifugation of the chambers before incubation.

6. Effects of cytochalasin B on polymorphonuclear leucocyte locomotion, phagocytosis and glycolysis.

7. Re-assessment of Boyden's technique for measuring chemotaxis.

8. Differences in superoxide production by nonmigrating and migrating human monocyte subpopulations.

9. Quantitative comparisons of various biological responses of neutrophils to different active and inactive chemotactic factors.

10. Leukocyte migration agarose test for the assessment of human neutrophil chemotaxis. II. Variables in the attraction assay.

1. Spatially directed guidance of stem cell population migration by immobilized patterns of growth factors.

2. Mycoplasma pulmonis possesses a novel chemoattractant for B lymphocytes.

3. Role of connective tissue growth factor in the retinal vasculature during development and ischemia.

4. Combined boyden-flow cytometry assay improves quantification and provides phenotypification of leukocyte chemotaxis.

5. Silencing of directional migration in roundabout4 knockdown endothelial cells.