Literature DB >> 6373202

Homologous in vitro transcription of linear DNA fragments containing the tRNAArg-tRNAAsp gene pair from Saccharomyces cerevisiae.

K Kjellin-Straby, D R Engelke, J Abelson.   

Abstract

Transcription of a tRNAArg-tRNAAsp gene pair from Saccharomyces cerevisiae by an homologous yeast extract results in a dimeric percursor molecule which is processed to mature-sized tRNAArg and tRNAAsp molecules. We have transcribed linear DNA fragments cleaved within the gene sequences to show that precursor synthesis is not dependent on the internal promoter of the second gene (tRNAAsp). Furthermore, the second gene does not support independent transcription when the normal upstream initiation site is removed.

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Year:  1984        PMID： 6373202     DOI： 10.1089/dna.1984.3.167

Source DB:  PubMed          Journal:  DNA        ISSN： 0198-0238


  7 in total

1.  Retrotransposon profiling of RNA polymerase III initiation sites.

Authors:  Xiaojie Qi; Kenneth Daily; Kim Nguyen; Haoyi Wang; David Mayhew; Paul Rigor; Sholeh Forouzan; Mark Johnston; Robi David Mitra; Pierre Baldi; Suzanne Sandmeyer
Journal:  Genome Res       Date:  2012-01-27       Impact factor: 9.043

2.  Mutational analysis of the coordinate expression of the yeast tRNAArg-tRNAAsp gene tandem.

Authors:  V M Reyes; A Newman; J Abelson
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272

3.  Expression and function of a human initiator tRNA gene in the yeast Saccharomyces cerevisiae.

Authors:  M A Francis; U L Rajbhandary
Journal:  Mol Cell Biol       Date:  1990-09       Impact factor: 4.272

4.  A yeast tRNA(Arg) gene can act as promoter for a 5' flank deficient, non-transcribable tRNA(SUP)6 gene to produce biologically active suppressor tRNA.

Authors:  K B Stråby
Journal:  Nucleic Acids Res       Date:  1988-04-11       Impact factor: 16.971

5.  Local features determine Ty3 targeting frequency at RNA polymerase III transcription start sites.

Authors:  Kurt Patterson; Farbod Shavarebi; Christophe Magnan; Ivan Chang; Xiaojie Qi; Pierre Baldi; Virginia Bilanchone; Suzanne B Sandmeyer
Journal:  Genome Res       Date:  2019-06-27       Impact factor: 9.043

6.  Expanding the genetic code of yeast for incorporation of diverse unnatural amino acids via a pyrrolysyl-tRNA synthetase/tRNA pair.

Authors:  Susan M Hancock; Rajendra Uprety; Alexander Deiters; Jason W Chin
Journal:  J Am Chem Soc       Date:  2010-10-27       Impact factor: 15.419

7.  In vivo modulation of yeast tRNA gene expression by 5'-flanking sequences.

Authors:  K C Raymond; G J Raymond; J D Johnson
Journal:  EMBO J       Date:  1985-10       Impact factor: 11.598
  7 in total

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