Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 DNA replication regulated by the priming promoter.

Literature DB >> 6369250

DNA replication regulated by the priming promoter.

Abstract

ColE1 derivative plasmids were constructed in which the natural promoter that primes replication or, in addition, the region coding for the RNA I control element had been deleted. In all of these molecules priming of the origin was effected by read-through transcription from constitutive or inducible (lacUV5) promoters inserted farther upstream. In the latter case, regulation of lac repressor activity with IPTG resulted in controlled plasmid levels in vivo. These results indicated that, at least in the absence of other control elements, regulation of the priming promoter was sufficient to control DNA replication and determine plasmid copy number.

Entities: Chemical

Mesh：

Substances：
Repressor Proteins

Year: 1984 PMID： 6369250 PMCID： PMC318696 DOI： 10.1093/nar/12.6.2641

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

14 in total

1. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors: J G Sutcliffe
Journal: Cold Spring Harb Symp Quant Biol Date: 1979

2. Mutants that make more lac repressor.

Authors: B Müller-Hill; L Crapo; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1968-04 Impact factor: 11.205

3. Mutational alteration of the maximal level of Lac operon expression.

Authors: J Scaife; J R Beckwith
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

4. Cruciform structures in supercoiled DNA.

Authors: N Panayotatos; R D Wells
Journal: Nature Date: 1981-02-05 Impact factor: 49.962

5. Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter.

Authors: G Cesareni; M A Muesing; B Polisky
Journal: Proc Natl Acad Sci U S A Date: 1982-10 Impact factor: 11.205

6. A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis.

Authors: R D Klein; E Selsing; R D Wells
Journal: Plasmid Date: 1980-01 Impact factor: 3.466

7. Specific deletion of DNA sequences between preselected bases.

Authors: N Panayotatos; K Truong
Journal: Nucleic Acids Res Date: 1981-11-11 Impact factor: 16.971

8. Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.

Authors: S Nagata; H Taira; A Hall; L Johnsrud; M Streuli; J Ecsödi; W Boll; K Cantell; C Weissmann
Journal: Nature Date: 1980-03-27 Impact factor: 49.962

9. Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript.

Authors: J Tomizawa; T Itoh
Journal: Proc Natl Acad Sci U S A Date: 1981-10 Impact factor: 11.205

10. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.

Authors: A Oka; N Nomura; M Morita; H Sugisaki; K Sugimoto; M Takanami
Journal: Mol Gen Genet Date: 1979-05-04

9 in total

DNA replication regulated by the priming promoter.

1. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

2. Mutants that make more lac repressor.

3. Mutational alteration of the maximal level of Lac operon expression.

4. Cruciform structures in supercoiled DNA.

5. Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter.

6. A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis.

7. Specific deletion of DNA sequences between preselected bases.

8. Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.

9. Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript.

10. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.

1. Methylation-dependent transcription controls plasmid replication of the CloDF13 cop-1(Ts) mutant.

2. A temperature-dependent pBR322 copy number mutant resulting from a Tn5 position effect.

3. Analysis of dominant copy number mutants of the plasmid pMB1.

Review 4. Modulation of ColE1-like plasmid replication for recombinant gene expression.

5. Synchronized DNA cycling across a bacterial population.

6. Cleavage within an RNase III site can control mRNA stability and protein synthesis in vivo.

Review 7. Replication of plasmids in gram-negative bacteria.

8. A plasmid system with tunable copy number.

Review 9. Tuning the dials of Synthetic Biology.