A mitochondrial ribosomal RNA mutation and its nuclear suppressors.

We have isolated cold-resistant revertants from a mitochondrial cold-sensitive mutation, cs909, localized in the 21S ribosomal RNA gene. Two types of revertants have been isolated: (1) strong revertants which were shown to be due to a single, nuclear, dominant suppressor; (2) weak revertants which are all due to the presence of a single, nuclear, recessive suppressor. The recessive suppressor, when separated from the mitochondrial mutation, itself confers a cold-sensitive phenotype, that is, there is a mutual suppression between the mitochondrial cold-sensitive mutation and the nuclear cold-sensitive mutation. The suppressor by itself produces modified ribosomes and therefore probably codes for an element of the mitochondrial ribosome such as a ribosomal protein.