Literature DB >> 6363715

Escherichia coli DNA photolyase is a flavoprotein.

A Sancar, G B Sancar.   

Abstract

Escherichia coli DNA photolyase (photoreactivating enzyme) was purified to homogeneity from a strain that greatly overproduces the protein. The purified enzyme has absorption peaks at 280 and 380 nm, a fluorescence emission peak at 480 nm and, upon denaturation, releases a chromophore that has the spectroscopic properties of flavin adenine dinucleotide (FAD), indicating that FAD is an intrinsic chromophore of the enzyme.

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Year:  1984        PMID： 6363715     DOI： 10.1016/s0022-2836(84)80040-6

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  22 in total

1.  Profile of Aziz Sancar.

Authors:  Nick Zagorski
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-01       Impact factor: 11.205

2.  Photoactivation of the flavin cofactor in Xenopus laevis (6 - 4) photolyase: observation of a transient tyrosyl radical by time-resolved electron paramagnetic resonance.

Authors:  Stefan Weber; Christopher W M Kay; Heike Mögling; Klaus Möbius; Kenichi Hitomi; Takeshi Todo
Journal:  Proc Natl Acad Sci U S A       Date:  2002-01-22       Impact factor: 11.205

3.  Photoreactivation in phr mutants of Escherichia coli K-12.

Authors:  I Husain; A Sancar
Journal:  J Bacteriol       Date:  1987-06       Impact factor: 3.490

4.  Molecular characterization of a gene encoding a photolyase from Streptomyces griseus.

Authors:  T Kobayashi; M Takao; A Oikawa; A Yasui
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971

5.  Characterization of a cold-adapted DNA photolyase from C. psychrerythraea 34H.

Authors:  Sudipto Munshi; Ananthi Rajamoorthi; Robert J Stanley
Journal:  Extremophiles       Date:  2017-07-19       Impact factor: 2.395

6.  The Roles of Several Residues of Escherichia coli DNA Photolyase in the Highly Efficient Photo-Repair of Cyclobutane Pyrimidine Dimers.

Authors:  Lei Xu; Guoping Zhu
Journal:  J Nucleic Acids       Date:  2010-08-31

7.  Time-resolved EPR studies with DNA photolyase: excited-state FADH0 abstracts an electron from Trp-306 to generate FADH-, the catalytically active form of the cofactor.

Authors:  S T Kim; A Sancar; C Essenmacher; G T Babcock
Journal:  Proc Natl Acad Sci U S A       Date:  1993-09-01       Impact factor: 11.205

8.  Sequence of the Saccharomyces cerevisiae PHR1 gene and homology of the PHR1 photolyase to E. coli photolyase.

Authors:  G B Sancar
Journal:  Nucleic Acids Res       Date:  1985-11-25       Impact factor: 16.971

Review 9.  Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae.

Authors:  E C Friedberg
Journal:  Microbiol Rev       Date:  1988-03

10.  The third chromophore of DNA photolyase: Trp-277 of Escherichia coli DNA photolyase repairs thymine dimers by direct electron transfer.

Authors:  S T Kim; Y F Li; A Sancar
Journal:  Proc Natl Acad Sci U S A       Date:  1992-02-01       Impact factor: 11.205
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