Literature DB >> 6363210

Transcriptional mapping of two yeast genes coding for glyceraldehyde 3-phosphate dehydrogenase isolated by sequence homology with the chicken gene.

A M Musti, Z Zehner, K A Bostian, B M Paterson, R A Kramer.   

Abstract

Homology between the coding regions of the chicken and yeast glyceraldehyde 3-phosphate dehydrogenase (GAPDH) genes was directly demonstrated by the hybridization of a cDNA clone coding for GAPDH in the chicken with EcoRI-digested yeast DNA. A yeast EcoRI fragment library in bacteriophage lambda was screened using the chicken cDNA plasmid as probe, and two recombinant phages were isolated, each one containing a different GAPDH gene. The initiation and termination sites for the GAPDH mRNA were localized for the two different GAPDH genes and compared to those of other yeast genes. Measurements of the relative mRNA levels for the two genes show that both genes are transcribed at about the same level when yeasts are grown on glucose media.

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Year:  1983        PMID： 6363210     DOI： 10.1016/0378-1119(83)90175-0

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  12 in total

1.  A temperature-sensitive lambda cI repressor functions on a modified operator in yeast cells by masking the TATA element.

Authors:  H Wedler; R Wambutt
Journal:  Mol Gen Genet       Date:  1995-08-30

2.  Multicopy CUP1 plasmids enhance cadmium and copper resistance levels in yeast.

Authors:  A Jeyaprakash; J W Welch; S Fogel
Journal:  Mol Gen Genet       Date:  1991-03

3.  Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene.

Authors:  J Y Tso; X H Sun; T H Kao; K S Reece; R Wu
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971

4.  Regulated expression of a human interferon gene in yeast: control by phosphate concentration or temperature.

Authors:  R A Kramer; T M DeChiara; M D Schaber; S Hilliker
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

5.  Expression enhancement of the Tn5 neomycin-resistance gene by removal of upstream ATG sequences and its use for probing heterologous upstream activating sequences in yeast.

Authors:  S Yagi; K Yagi-Tanaka; J Yoshioka; M Suzuki
Journal:  Curr Genet       Date:  1993 Jul-Aug       Impact factor: 3.886

6.  Trifluoperazine inhibits insulin action on glucose metabolism in fat cells without affecting inhibition of lipolysis.

Authors:  Y Shechter
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

7.  The chromatin structure at the promoter of a glyceraldehyde phosphate dehydrogenase gene from Saccharomyces cerevisiae reflects its functional state.

Authors:  B Pavlović; W Hörz
Journal:  Mol Cell Biol       Date:  1988-12       Impact factor: 4.272

8.  Saccharomyces cerevisiae exhibits a sporulation-specific temporal pattern of transcript accumulation.

Authors:  D B Kaback; L R Feldberg
Journal:  Mol Cell Biol       Date:  1985-04       Impact factor: 4.272

9.  The CUP2 gene product regulates the expression of the CUP1 gene, coding for yeast metallothionein.

Authors:  J Welch; S Fogel; C Buchman; M Karin
Journal:  EMBO J       Date:  1989-01       Impact factor: 11.598

10.  A glyceraldehyde-3-phosphate dehydrogenase pseudogene on the short arm of the human X chromosomes defines a multigene family.

Authors:  F J Benham; S Hodgkinson; K E Davies
Journal:  EMBO J       Date:  1984-11       Impact factor: 11.598
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