Literature DB >> 6360965

Demonstration of protease isoenzymes by two-dimensional polyacrylamide electrophoresis.

P K Sinha, R Gossrau.   

Abstract

A two-dimensional polyacrylamide electrophoresis system is described for the detection of protease isoenzymes using 4-methoxy-2-naphthylamine derivates as substrates. With this technique is possible to detect isoenzymes differing in molecular weight by separation according to size and shape. This is not possible by the use of isoelectric focusing alone.

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Year:  1983        PMID： 6360965     DOI： 10.1007/bf00491782

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  7 in total

1.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

2.  Preparation of delipidized serum protein for use in cell culture systems.

Authors:  G H Rothblat; L Y Arbogast; L Ouellette; B V Howard
Journal:  In Vitro       Date:  1976-08

3.  Contributions of histochemistry to the development of the proteolytic enzyme detection system in diagnostic medicine.

Authors:  R E Smith
Journal:  J Histochem Cytochem       Date:  1983-01       Impact factor: 2.479

4.  The usefulness of the analytical electrofocusing in a thin-layer polyacrylamide gel (PAG) in the histochemistry of enzymes cleaving peptide bonds.

Authors:  Z Lojda; J Kulich
Journal:  Histochemistry       Date:  1981

5.  Investigation of proteases with chromogenic substrates after isoelectric focusing (IEF).

Authors:  P Sinha; R Gossrau
Journal:  Histochemistry       Date:  1984

6.  Isoelectric focusing (IEF) and band detection with fluorogenic protease substrates.

Authors:  P Sinha; R Gossrau
Journal:  Histochemistry       Date:  1984

7.  Fluorescence detection of proteases with AFC, AMC and MNA peptides using isoelectric focusing.

Authors:  P Sinha; R Gossrau; R E Smith; Z Lojda
Journal:  Adv Exp Med Biol       Date:  1984       Impact factor: 2.622
  7 in total
  2 in total

1.  Investigation of proteases with chromogenic substrates after isoelectric focusing (IEF).

Authors:  P Sinha; R Gossrau
Journal:  Histochemistry       Date:  1984

Review 2.  Cytochemistry of membrane proteases.

Authors:  R Gossrau
Journal:  Histochem J       Date:  1985-07
  2 in total

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