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Literature DB >> 6358197

Transfer of pRD1 to Pseudomonas syringae and evidence for its integration into the chromosome.

Abstract

Plasmid pRD1 was conjugatively transferred from Escherichia coli to Pseudomonas syringae. Subculturing the transconjugate on a medium that selected for pRD1-determined His+ Kmr resulted in the loss of pRD1 as an extrachromosomal element as detected by agarose gel electrophoresis. DNA hybridization provided evidence for the integration of pRD1 into the P. syringae chromosome.

Entities: Chemical Species

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Year: 1983 PMID： 6358197 PMCID： PMC217987 DOI： 10.1128/jb.156.3.1349-1351.1983

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

7 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

3. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors: G M Wahl; M Stern; G R Stark
Journal: Proc Natl Acad Sci U S A Date: 1979-08 Impact factor: 11.205

2 in total

1. Sym plasmid genes of Rhizobium trifolii expressed in Lignobacter and Pseudomonas strains.

Authors: J Plazinski; B G Rolfe
Journal: J Bacteriol Date: 1985-06 Impact factor: 3.490

2. High-Quality Draft Genome Sequence of Pseudomonas syringae pv. Syringae Strain SM, Isolated from Wheat.

Authors: Alexey Dudnik; Robert Dudler
Journal: Genome Announc Date: 2013-08-15

2 in total

Transfer of pRD1 to Pseudomonas syringae and evidence for its integration into the chromosome.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

3. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

4. Construction of a P plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae.

5. Rapid procedure for detection and isolation of large and small plasmids.

6. F'-plasmid transfer from Escherichia coli to Pseudomonas fluorescens.

7. Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized.

1. Sym plasmid genes of Rhizobium trifolii expressed in Lignobacter and Pseudomonas strains.

2. High-Quality Draft Genome Sequence of Pseudomonas syringae pv. Syringae Strain SM, Isolated from Wheat.