Literature DB >> 384162

Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized.

F C Cannon, G E Riedel, F M Ausubel.   

Abstract

A HindIII (17.0 kb) and an EcoR1 restriction fragment (6.9 kb) of Klebsiella pneumoniae nif DNA were cloned on two small amplifiable plasmids, pCM1 and pSA30 respectively. These plasmids between them carry 14 of the 15 known Klebsiella nif genes. The operon for the three structural genes for nitrogenase, nifpHDK, is carried on pSA30: four and five of the remaining six operons are on pCRA37 and pCM1 respectively. All of the nif genes were assigned to endonuclease restriction fragments of DNA using the Southern blotting technique (Southern, 1975) with total DNA of nif insertion mutants and radioactive plasmid DNA which contained cloned nif DNA sequences. Their locations were consistent with the genetic map of nif genes. The estimated size of the nif gene cluster was 24 kb.

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Year:  1979        PMID： 384162     DOI： 10.1007/bf00433306

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  17 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Mutants of Escherichia coli requiring methionine or vitamin B12.

Authors:  B D DAVIS; E S MINGIOLI
Journal:  J Bacteriol       Date:  1950-07       Impact factor: 3.490

3.  Cloning nitrogen fixing genes from Klebsiella pneumoniae in vitro and the isolation of NIF promoter mutants affecting glutamine synthetase regulation.

Authors:  F Ausubel; G Riedel; F Cannon; A Peskin; R Margolskee
Journal:  Basic Life Sci       Date:  1977

4.  Genetic and biochemical analysis of mutants induced by bacteriophage Mu DNA integration into Klebsiella pneumoniae nitrogen fixation genes.

Authors:  C Elmerich; J Houmard; L Sibold; I Manheimer; N Charpin
Journal:  Mol Gen Genet       Date:  1978-10-04

5.  The nitrogen fixation genes.

Authors:  S L Streicher; E G Gurney; R C Valentine
Journal:  Nature       Date:  1972-10-27       Impact factor: 49.962

6.  A simple method for the preparation of large quantities of pure plasmid DNA.

Authors:  G O Humphreys; G A Willshaw; E S Anderson
Journal:  Biochim Biophys Acta       Date:  1975-04-02

7.  Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteins.

Authors:  R R Eady; B E Smith; K A Cook; J R Postgate
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

8.  Physical map of chromosomal nitrogen fixation (nif) genes of Klebsiella pneumoniae.

Authors:  G E Riedel; F M Ausubel; F C Cannon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

9.  Complementation analysis of Klebsiella pneumoniae mutants defective in nitrogen fixation.

Authors:  R Dixon; C Kennedy; A Kondorosi; V Krishnapillai; M Merrick
Journal:  Mol Gen Genet       Date:  1977-11-29

10.  Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae.

Authors:  T MacNeil; D MacNeil; G P Roberts; M A Supiano; W J Brill
Journal:  J Bacteriol       Date:  1978-10       Impact factor: 3.490
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  65 in total

1.  Isolation and Characterization of Marine Caulobacters and Assessment of Their Potential for Genetic Experimentation.

Authors:  Nick Anast; John Smit
Journal:  Appl Environ Microbiol       Date:  1988-03       Impact factor: 4.792

2.  Organization and expression of Rhizobium meliloti nitrogen fixation genes.

Authors:  D Corbin; L Barran; G Ditta
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205

3.  Construction of new yeast vectors and cloning of the nif (nitrogen fixation) gene cluster of Klebsiella pneumoniae in yeast.

Authors:  C Gerbaud; C Elmerich; N Tandeau de Marsac; P Chocat; N Charpin; M Guérineau; J P Aubert
Journal:  Curr Genet       Date:  1981-07       Impact factor: 3.886

4.  Cloning of the entire region for nitrogen fixation from Klebsiella pneumoniae on a multicopy plasmid vehicle in Escherichia coli.

Authors:  A Pühler; H J Burkardt; W Klipp
Journal:  Mol Gen Genet       Date:  1979-10-02

5.  Stability of Bradyrhizobium japonicum Inoculants after Introduction into Soil.

Authors:  B Brunel; J C Cleyet-Marel; P Normand; R Bardin
Journal:  Appl Environ Microbiol       Date:  1988-11       Impact factor: 4.792

6.  Examination of Genetic Relatedness of Marine Synechococcus spp. by Using Restriction Fragment Length Polymorphisms.

Authors:  S E Douglas; N Carr
Journal:  Appl Environ Microbiol       Date:  1988-12       Impact factor: 4.792

7.  Isolation and Characterization of Frankia sp. Strain FaC1 Genes Involved in Nitrogen Fixation.

Authors:  J M Ligon; J P Nakas
Journal:  Appl Environ Microbiol       Date:  1987-10       Impact factor: 4.792

8.  Isolation of transfer-negative nif-plasmids (pCE1) and their integration into the chromosome of Escherichia coli with the help of phage Mu.

Authors:  M Göttfert; W Klingmüller
Journal:  Mol Gen Genet       Date:  1985

9.  N2 fixation in marine heterotrophic bacteria: dynamics of environmental and molecular regulation.

Authors:  J A Coyer; A Cabello-Pasini; H Swift; R S Alberte
Journal:  Proc Natl Acad Sci U S A       Date:  1996-04-16       Impact factor: 11.205

10.  DNA hybridization analysis of the nif region of two methylotrophs and molecular cloning of nif-specific DNA.

Authors:  A E Toukdarian; M E Lidstrom
Journal:  J Bacteriol       Date:  1984-03       Impact factor: 3.490
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