Literature DB >> 6358039

Enterobacterial common antigen-tetanus toxoid conjugate as immunogen.

C Lugowski, M Kułakowska, E Romanowska.   

Abstract

The methods of limited periodate oxidation and reductive amination were used to obtain covalently linked enterobacterial common antigen (ECA) with tetanus toxoid. This procedure is simple and gives a good yield of the conjugate with high ECA content (molecular ratio of ECA to tetanus toxoid, 6:1). The ECA-tetanus toxoid conjugate is immunogenic in rabbits, in contrast to free ECA or a mixture of ECA with proteins. This conjugate produces high levels of ECA-specific immunoglobulin G antibodies, which can be used as a standard serum.

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Year:  1983        PMID： 6358039      PMCID： PMC264411          DOI： 10.1128/iai.42.3.1086-1091.1983

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  29 in total

1.  Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.

Authors:  E Engvall; K Jonsson; P Perlmann
Journal:  Biochim Biophys Acta       Date:  1971-12-28

2.  Colorimetric differentiation of hexosamines.

Authors:  J Ludowieg; J D Benmaman
Journal:  Anal Biochem       Date:  1967-04       Impact factor: 3.365

3.  Role of T lymphocytes in the humoral immune response. I. Proliferation of B lymphocytes in thymus-deprived mice.

Authors:  J M Davie; W E Paul
Journal:  J Immunol       Date:  1974-11       Impact factor: 5.422

4.  Augmented anti-S 3 antibody responses to an S 3 -protein conjugate.

Authors:  W E Paul; D H Katz; B Benacerraf
Journal:  J Immunol       Date:  1971-09       Impact factor: 5.422

5.  An immunogenic polysaccharide-protein conju- gate.

Authors:  R J Fielder; C T Bishop; S F Grappel; F Blank
Journal:  J Immunol       Date:  1970-07       Impact factor: 5.422

6.  The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.

Authors:  S Avrameas; T Ternynck
Journal:  Immunochemistry       Date:  1969-01

7.  Crossed immunoelectrophoresis.

Authors:  B Weeke
Journal:  Scand J Immunol Suppl       Date:  1973

8.  Characterization of rabbit fast and slow 6-6S IgG.

Authors:  W P Faulk; K W Pondman
Journal:  Immunochemistry       Date:  1969-03

9.  The nature of crosslinking in collagens from mineralized tissues.

Authors:  G Mechanic; P M Gallop; M L Tanzer
Journal:  Biochem Biophys Res Commun       Date:  1971-11-05       Impact factor: 3.575

10.  Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors:  P Cuatrecasas
Journal:  J Biol Chem       Date:  1970-06       Impact factor: 5.157
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  2 in total

1.  First evidence for a covalent linkage between enterobacterial common antigen and lipopolysaccharide in Shigella sonnei phase II ECALPS.

Authors:  Tomasz K Gozdziewicz; Czeslaw Lugowski; Jolanta Lukasiewicz
Journal:  J Biol Chem       Date:  2013-12-09       Impact factor: 5.157

Review 2.  Enterobacterial Common Antigen: Synthesis and Function of an Enigmatic Molecule.

Authors:  Ashutosh K Rai; Angela M Mitchell
Journal:  mBio       Date:  2020-08-11       Impact factor: 7.867
  2 in total

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