Literature DB >> 6353356

Determination of glutathione in biological material by high pressure liquid chromatography.

A J Baars, D D Breimer.   

Abstract

A rapid and selective determination of reduced glutathione in biological material is described, based on its conjugation with 1-chloro-2,4-dinitrobenzene. The reaction product has a UV absorption maximum at 340 nm and is analysed by reversed phase high pressure liquid chromatography. Linear calibration graphs were obtained in the concentration range between 50 microM and 2 mM glutathione in standard solutions and in biological material (rat liver and bacterial homogenates). The detection limit is about 2 microM glutathione when using 20 microliters injection samples.

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Year:  1983        PMID： 6353356     DOI： 10.1007/bf01961471

Source DB:  PubMed          Journal:  Pharm Weekbl Sci        ISSN： 0167-6555


  8 in total

1.  Tissue sulfhydryl groups.

Authors:  G L ELLMAN
Journal:  Arch Biochem Biophys       Date:  1959-05       Impact factor: 4.013

2.  A fluorometric method for determination of oxidized and reduced glutathione in tissues.

Authors:  P J Hissin; R Hilf
Journal:  Anal Biochem       Date:  1976-07       Impact factor: 3.365

Review 3.  The glutathione S-transferases: a group of multifunctional detoxification proteins.

Authors:  W B Jakoby
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1978

4.  Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.

Authors:  W H Habig; M J Pabst; W B Jakoby
Journal:  J Biol Chem       Date:  1974-11-25       Impact factor: 5.157

Review 5.  The glutathione S-transferases: their role in detoxification and toxification of xenobiotics.

Authors:  A J Baars; D D Breimer
Journal:  Ann Biol Clin (Paris)       Date:  1980       Impact factor: 0.459

6.  The relation between the structure of vicinal dihalogen compounds and their mutagenic activation via conjugation to glutathione.

Authors:  P J van Bladeren; D D Breimer; G M Rotteveel-Smijs; P de Knijff; G R Mohn; B van Meeteren-Wälchli; W Buijs; A van der Gen
Journal:  Carcinogenesis       Date:  1981       Impact factor: 4.944

7.  Estimation of glutathione in rat liver by reversed-phase high-performance liquid chromatography: separation from cysteine and gamma-glutamylcysteine.

Authors:  J Reeve; J Kuhlenkamp; N Kaplowitz
Journal:  J Chromatogr       Date:  1980-06-27

8.  Effects of variation in the dietary supply of cysteine and methionine on liver concentration of glutathione and "active sulfate" (PAPS) and serum levels of sulfate, cystine, methionine and taurine: relation to the metabolism of acetaminophen.

Authors:  E J Glazenburg; I M Jekel-Halsema; E Scholtens; A J Baars; G J Mulder
Journal:  J Nutr       Date:  1983-07       Impact factor: 4.798
  8 in total

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