Literature DB >> 6350328

Aequorin measurements of free calcium in single mammalian cells.

P H Cobbold, K S Cuthbertson, M H Goyns, V Rice.   

Abstract

The calcium-triggered photoprotein aequorin has been used as a sensitive indicator of cytoplasmic free calcium in cells of relatively large size. We describe here several novel modifications to the technique that make it possible to detect resting levels of free calcium in single mammalian oocytes, fibroblasts and granulosa cells. Concentrations of free calcium as low as 1 X 10(-7) M have been measured in single fibroblasts, corresponding to a detection limit of 10(-18)mol.

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Year:  1983        PMID： 6350328     DOI： 10.1242/jcs.61.1.123

Source DB:  PubMed          Journal:  J Cell Sci        ISSN： 0021-9533            Impact factor:   5.285


  8 in total

1.  Formation of the Ca2+-activated photoprotein obelin from apo-obelin and mRNA inside human neutrophils.

Authors:  A K Campbell; A K Patel; Z S Razavi; F McCapra
Journal:  Biochem J       Date:  1988-05-15       Impact factor: 3.857

2.  Cytosolic free Ca2+ in single rat heart cells during anoxia and reoxygenation.

Authors:  A Allshire; H M Piper; K S Cuthbertson; P H Cobbold
Journal:  Biochem J       Date:  1987-06-01       Impact factor: 3.857

Review 3.  Fluorescence and bioluminescence measurement of cytoplasmic free calcium.

Authors:  P H Cobbold; T J Rink
Journal:  Biochem J       Date:  1987-12-01       Impact factor: 3.857

Review 4.  Use of photoproteins as intracellular calcium indicators.

Authors:  J R Blinks
Journal:  Environ Health Perspect       Date:  1990-03       Impact factor: 9.031

5.  Free calcium transients in chemotactic and non-chemotactic strains of Escherichia coli determined by using recombinant aequorin.

Authors:  N J Watkins; M R Knight; A J Trewavas; A K Campbell
Journal:  Biochem J       Date:  1995-03-15       Impact factor: 3.857

6.  Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes.

Authors:  N M Woods; K S Cuthbertson; P H Cobbold
Journal:  Biochem J       Date:  1987-09-15       Impact factor: 3.857

7.  Rapid transient elevations of cytosolic calcium triggered by thyrotropin releasing hormone in individual cells of the pituitary line GH3B6.

Authors:  B P Winiger; W Schlegel
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

8.  Subcellular calcium measurements in mammalian cells using jellyfish photoprotein aequorin-based probes.

Authors:  Massimo Bonora; Carlotta Giorgi; Angela Bononi; Saverio Marchi; Simone Patergnani; Alessandro Rimessi; Rosario Rizzuto; Paolo Pinton
Journal:  Nat Protoc       Date:  2013-10-10       Impact factor: 13.491
  8 in total

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