Literature DB >> 6345196

Quantitative study of interaction of deacylated tRNA with Escherichia coli ribosomes. Role of 50 S subunits in formation of the E site.

S V Kirillov, E M Makarov.   

Abstract

The 30 S subunit contains 2 sites for tRNA binding (Phe-tRNA, AcPhe-tRNA, tRNAPheOH) with the functional properties of D and A sites of the 70 S ribosome after attachment of 50 S subunit. The third (E) site specific for deacylated tRNA is introduced into 70 S ribosome by its 50 S subunit. The E-site binding of tRNAPheOH is not sensitive to either tetracycline and edeine, and practically codon-independent. The affinity constant of tRNAPheOH for the E site is 2-3 orders of magnitude lower than that for the D site.

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Year:  1983        PMID： 6345196     DOI： 10.1016/0014-5793(83)81122-3

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  18 in total

1.  Interactions between 23S rRNA and tRNA in the ribosomal E site.

Authors:  M Bocchetta; L Xiong; S Shah; A S Mankin
Journal:  RNA       Date:  2001-01       Impact factor: 4.942

2.  Photolabile anticodon stem-loop analogs of tRNAPhe as probes of ribosomal structure and structural fluctuation at the decoding center.

Authors:  Zhanna Druzina; Barry S Cooperman
Journal:  RNA       Date:  2004-08-30       Impact factor: 4.942

3.  Destabilization of the P site codon-anticodon helix results from movement of tRNA into the P/E hybrid state within the ribosome.

Authors:  Kevin G McGarry; Sarah E Walker; Huanyu Wang; Kurt Fredrick
Journal:  Mol Cell       Date:  2005-11-23       Impact factor: 17.970

4.  How are tRNAs and mRNA arranged in the ribosome? An attempt to correlate the stereochemistry of the tRNA-mRNA interaction with constraints imposed by the ribosomal topography.

Authors:  V Lim; C Venclovas; A Spirin; R Brimacombe; P Mitchell; F Müller
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

5.  Messenger RNA conformations in the ribosomal E site revealed by X-ray crystallography.

Authors:  Lasse Jenner; Bernard Rees; Marat Yusupov; Gulnara Yusupova
Journal:  EMBO Rep       Date:  2007-08-03       Impact factor: 8.807

6.  Ribosome binding of DNA analogs of tRNA requires base modifications and supports the "extended anticodon".

Authors:  V Dao; R Guenther; A Malkiewicz; B Nawrot; E Sochacka; A Kraszewski; J Jankowska; K Everett; P F Agris
Journal:  Proc Natl Acad Sci U S A       Date:  1994-03-15       Impact factor: 11.205

7.  The "allosteric three-site model" of elongation cannot be confirmed in a well-defined ribosome system from Escherichia coli.

Authors:  Y P Semenkov; M V Rodnina; W Wintermeyer
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-29       Impact factor: 11.205

8.  Influence of systematic error on the shape of the scatchard plot of tRNAPhe binding to eukaryotic ribosomes.

Authors:  S A Nekhai; V E Beletzkij; D M Graifer
Journal:  Biochem J       Date:  1997-07-15       Impact factor: 3.857

9.  Calculation of the relative geometry of tRNAs in the ribosome from directed hydroxyl-radical probing data.

Authors:  S Joseph; M L Whirl; D Kondo; H F Noller; R B Altman
Journal:  RNA       Date:  2000-02       Impact factor: 4.942

10.  Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit.

Authors:  T Martin Schmeing; Peter B Moore; Thomas A Steitz
Journal:  RNA       Date:  2003-11       Impact factor: 4.942
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