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Literature DB >> 6344079

Consistent association between sigma elements and tRNA genes in yeast.

Abstract

Sigma is a recently described family of transposable elements in yeast (Saccharomyces cerevisiae). The most striking feature of the seven sigma elements that have been previously identified is that all are located 16-18 base pairs upstream from tRNA-encoding regions. Because these cases were all encountered in the process of studying specific tRNA genes, the full extent of the association between sigma elements and tRNA genes could not be assessed. In this paper, we report a more global characterization of the sigma family in a typical laboratory yeast strain: of the 30 copies of sigma that we estimate to be present in the haploid genome, we have cloned and analyzed 25 loci. Although in two cases a pair of sigma elements were found within several kilobases of each other, the majority occur as individual elements at widely dispersed sites. Moreover, in all 25 cases analyzed, the sigma elements are closely associated with tRNA genes. Thus, the sigma transposable element has been shown to have an absolute association with another gene family.

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Year: 1983 PMID： 6344079 PMCID： PMC394027 DOI： 10.1073/pnas.80.11.3292

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

16 in total

Consistent association between sigma elements and tRNA genes in yeast.

1. Gel electrophoresis of restriction fragments.

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

3. Cloning of the yeast tyrosine transfer RNA genes in bacteriophage lambda.

4. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

5. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae.

6. Identification of the yeast DNA sequences that correspond to specific tyrosine-inserting nonsense suppressor loci.

7. Reactions at the termini of tRNA with T4 RNA ligase.

8. Evidence for transposition of dispersed repetitive DNA families in yeast.

9. The origins of gene instability in yeast.

10. Analysis of chromosomal integration and deletions of yeast plasmids.

1. Transfer RNA genes are genomic targets for de Novo transposition of the yeast retrotransposon Ty3.

2. Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein.

Review 3. Integration site selection by retroviruses and transposable elements in eukaryotes.

4. Sigma elements are position-specific for many different yeast tRNA genes.

5. Frameshift suppressor mutations outside the anticodon in yeast proline tRNAs containing an intervening sequence.

6. Ylt1, a highly repetitive retrotransposon in the genome of the dimorphic fungus Yarrowia lipolytica.

7. Host sequences flanking the human T-cell leukemia virus type 1 provirus in vivo.

8. tau, a repeated DNA sequence in yeast.

9. The yeast repeated element sigma contains a hormone-inducible promoter.

10. A heat shock element in the phosphoglycerate kinase gene promoter of yeast.