Literature DB >> 6343424

Fluorescence immunoassay and passive latex agglutination as alternatives to hemagglutination inhibition for determining rubella immune status.

S L Fayram, A Nakasone, S Aarnaes, M Zartarian, E M Peterson, L M de la Maza.   

Abstract

Three different assays for detection of rubella antibodies, hemagglutination inhibition (HAI), fluorescence immunoassay (FIA), and passive latex agglutination (PLA) were used to test 297 human serum samples. Overall agreements for immune status were as follows: HAI versus FIA, 95.3% (283 of 297); HAI versus PLA (1:10 dilution), 96.3% (286 of 297); HAI versus PLA (undiluted), 93.9% (279 of 297); PLA (1:10 dilution) versus FIA, 94.9% (282 of 297); and PLA (undiluted) versus FIA, 97.9% (291 of 297). The HAI test is the most time consuming, subjective, and technically difficult to perform. The FIA and PLA tests are very rapid and less labor intensive. In addition, the FIA offers an objective determination of the patient's rubella antibody level.

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Year:  1983        PMID： 6343424      PMCID： PMC272717          DOI： 10.1128/jcm.17.4.685-688.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  False positive rubella virus haemagglutination inhibition reactions: occurrence and disclosure.

Authors:  G Haukenes; H Blom
Journal:  Med Microbiol Immunol       Date:  1975       Impact factor: 3.402

2.  Problems of spectrum and bias in evaluating the efficacy of diagnostic tests.

Authors:  D F Ransohoff; A R Feinstein
Journal:  N Engl J Med       Date:  1978-10-26       Impact factor: 91.245

3.  Comparison of the hemagglutination inhibition test and an indirect fluorescent-antibody test for detection of antibody to rubella virus in human sera.

Authors:  N E Cremer; S J Hagens; C Cossen
Journal:  J Clin Microbiol       Date:  1980-06       Impact factor: 5.948

4.  Detection of rubella antibodies by hemagglutination inhibition, indirect fluorescent-antibody test, and enzyme-linked immunosorbent assay.

Authors:  M V Zartarian; G Friedly; E M Peterson; L M de la Maza
Journal:  J Clin Microbiol       Date:  1981-12       Impact factor: 5.948

5.  Current status of rubella testing: a report based on data from the College of American Pathologists' surveys, 1978-1980.

Authors:  L P Skendzel
Journal:  Am J Clin Pathol       Date:  1981-10       Impact factor: 2.493

6.  Nonspecific reactions in the hemagglutination inhibition test for detection of rubella antibodies.

Authors:  D B Budzko; D F Jelinek; B W Wilcke
Journal:  J Clin Microbiol       Date:  1981-05       Impact factor: 5.948

7.  Variables of the rubella hemagglutination-inhibition test system and their effect on antigen and antibody titers.

Authors:  N J Schmidt; E H Lennette
Journal:  Appl Microbiol       Date:  1970-03

8.  A direct enzyme-linked immunosorbent assay (ELISA) for detection of antibodies for rubella virus in human sera.

Authors:  T J Cleary; A Cid; B Ellis; H Malkus; T Noto; S Halbert; A Castro
Journal:  Res Commun Chem Pathol Pharmacol       Date:  1978-02
  8 in total
  4 in total

1.  Evaluation of a latex agglutination test for screening antibodies to rubella virus.

Authors:  P León; F de Ory; C Domingo; J A López; J M Echevarría
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-04       Impact factor: 3.267

2.  Determination of immune status in patients with low antibody titers for rubella virus.

Authors:  S L Fayram; S Akin; S L Aarnaes; E M Peterson; L M de la Maza
Journal:  J Clin Microbiol       Date:  1987-01       Impact factor: 5.948

3.  Comparison of a simple latex agglutination test with hemolysis-in-gel, hemagglutination inhibition, and radioimmunoassay for detection of rubella virus antibodies.

Authors:  P Väänänen; V M Häivä; P Koskela; O Meurman
Journal:  J Clin Microbiol       Date:  1985-05       Impact factor: 5.948

4.  Comparison of five methods for the determination of rubella immunity.

Authors:  R L Sautter; A E Crist; L M Johnson; W D Lebar
Journal:  Infect Dis Obstet Gynecol       Date:  1994
  4 in total

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