Literature DB >> 6339641

Simple rapid methods for freezing hybridomas in 96-well microculture plates.

D E Wells, P J Price.   

Abstract

Macroscopic hybridoma colonies were frozen and recovered in a good state of viability in 96-well microculture plates using 2 freezing procedures. These methods offer convenient and rapid means of preserving hybridomas and will permit laboratories developing monoclonal antibodies to distribute workloads to more manageable levels without discarding possibly valuable hybridomas.

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Year:  1983        PMID: 6339641     DOI: 10.1016/0022-1759(83)90144-8

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  2 in total

1.  Rapid cryopreservation of five mammalian and one mosquito cell line at -80 degrees C while attached to flasks in a serum free cryopreservative.

Authors:  Joe Corsini; Ethan Mann
Journal:  Biol Proced Online       Date:  2005-03-14       Impact factor: 3.244

2.  Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation.

Authors:  Martin I Daily; Thomas F Whale; Riitta Partanen; Alexander D Harrison; Peter Kilbride; Stephen Lamb; G John Morris; Helen M Picton; Benjamin J Murray
Journal:  Cryobiology       Date:  2020-02-21       Impact factor: 2.487

  2 in total

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