Literature DB >> 6339372

Pyruvate promotion of DNA synthesis in serum-free primary cultures of adult rat hepatocytes.

J A McGowan, N L Bucher.   

Abstract

Pyruvate (2 to 60 mM), acting alone and in conjunction with insulin and epidermal growth factor (EGF), enhances DNA synthesis in primary monolayer cultures of adult rat liver parenchymal cells. Lactate can replace pyruvate in stimulating DNA synthesis. Several other intermediary metabolites (oxaloacetate, alpha-ketoglutarate, alpha-ketobutyrate, succinate, fumarate, and malate), though less potent than pyruvate and lactate, also elevate DNA synthesis, whereas alanine at similar concentrations is inhibitory.

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Year:  1983        PMID： 6339372     DOI： 10.1007/bf02618054

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  22 in total

1.  Primary culture of parenchymal liver cells on collagen membranes. Morphological and biochemical observations.

Authors:  G Michalopoulos; H C Pitot
Journal:  Exp Cell Res       Date:  1975-08       Impact factor: 3.905

2.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

3.  Hormonal regulation of DNA synthesis in primary cultures of adult rat hepatocytes: action of glucagon.

Authors:  D L Friedman; T H Claus; S J Pilkis; G E Pine
Journal:  Exp Cell Res       Date:  1981-10       Impact factor: 3.905

4.  Amino acid and energy requirements for rat hepatocytes in primary culture.

Authors:  P E Schwarze; A E Solheim; P O Seglen
Journal:  In Vitro       Date:  1982-01

5.  Induction of mitosis in primary cultures of adult rat hepatocytes under serum-free conditions.

Authors:  K Hasegawa; K Watanabe; M Koga
Journal:  Biochem Biophys Res Commun       Date:  1982-01-15       Impact factor: 3.575

6.  Oxocarboxylic acids, pyridine nucleotide-linked oxidoreductases and serum factors in regulation of cell proliferation.

Authors:  W L McKeehan; K A McKeehan
Journal:  J Cell Physiol       Date:  1979-10       Impact factor: 6.384

7.  L-Lactate or pyruvate stimulated growth of Novikoff rat hepatoma cells.

Authors:  L S Siegel; R W Bernlohr
Journal:  In Vitro       Date:  1979-07

8.  Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes.

Authors:  R A Richman; T H Claus; S J Pilkis; D L Friedman
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

9.  Rat hepatocyte primary cultures. IV. Maintenance in defined medium and the role of production of plasminogen activator and other proteases.

Authors:  G M Williams; E Bermudez; R H San; P J Goldblatt; M F Laspia
Journal:  In Vitro       Date:  1978-10

10.  Connective tissue biomatrix: its isolation and utilization for long-term cultures of normal rat hepatocytes.

Authors:  M Rojkind; Z Gatmaitan; S Mackensen; M A Giambrone; P Ponce; L M Reid
Journal:  J Cell Biol       Date:  1980-10       Impact factor: 10.539
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  12 in total

1.  Attachment and multiplication, morphology and protein production of human fetal primary liver cells cultured in hormonally defined media.

Authors:  M Salas-Prato; J F Tanguay; Y Lefebvre; D Wojciechowicz; H H Liem; D W Barnes; G Ouellette; U Muller-Eberhard
Journal:  In Vitro Cell Dev Biol       Date:  1988-03

2.  Purification and characterization of a growth factor from rat platelets for mature parenchymal hepatocytes in primary cultures.

Authors:  T Nakamura; H Teramoto; A Ichihara
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

3.  Influence of hormones and growth factors on viability, DNA, and protein content of adult hepatocytes in primary culture.

Authors:  K Wirthensohn; C A Barth
Journal:  In Vitro Cell Dev Biol       Date:  1985-10

4.  Epidermal growth factor and proliferation in rat hepatocytes in primary culture isolated at different times after partial hepatectomy.

Authors:  A Francavilla; P Ove; L Polimeno; C Sciascia; M L Coetzee; T E Starzl
Journal:  Cancer Res       Date:  1986-03       Impact factor: 12.701

5.  A simple medium for the study of hepatocyte growth in culture under defined conditions.

Authors:  T E Sand; T Christoffersen
Journal:  In Vitro Cell Dev Biol       Date:  1988-10

6.  Effect of cimetidine, ranitidine, famotidine and omeprazole on hepatocyte proliferation in vitro.

Authors:  A Francavilla; C Panella; L Polimeno; A Di Leo; L Makowka; M Barone; A Amoruso; M Ingrosso; T E Starzl
Journal:  J Hepatol       Date:  1989-01       Impact factor: 25.083

7.  Estrogen-dependent DNA synthesis in cultures of Xenopus liver parenchymal cells.

Authors:  B S Aprison; L Martin-Morris; R J Spolski; L J Wangh
Journal:  In Vitro Cell Dev Biol       Date:  1986-08

8.  CCAAT/enhancer-binding protein (C/EBP) immunoreactivity during rat liver carcinogenesis.

Authors:  E Skarpen; B Lindeman; G H Thoresen; M Låg; T Christoffersen; H S Huitfeldt
Journal:  Histochem Cell Biol       Date:  1995-10       Impact factor: 4.304

9.  The bicarbonate ion is essential for efficient DNA synthesis by primary cultured rat hepatocytes.

Authors:  T Mitaka; G L Sattler; H C Pitot
Journal:  In Vitro Cell Dev Biol       Date:  1991-07

10.  Different response to epidermal growth factor of hepatocytes in cultures isolated from male or female rat liver. Inhibitor effect of estrogen on binding and mitogenic effect of epidermal growth factor.

Authors:  A Francavilla; P Ove; L Polimeno; C Sciascia; M Coetzee; R Pellici; S Todo; I Kam; T E Starzl
Journal:  Gastroenterology       Date:  1987-09       Impact factor: 22.682
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