Literature DB >> 3053597

A simple medium for the study of hepatocyte growth in culture under defined conditions.

T E Sand1, T Christoffersen.   

Abstract

The combination (1:1) of Dulbecco's modified Eagle's medium and Waymouth's medium MAB 87/3 was found to provide favorable conditions for serum-free culture and growth of adult rat hepatocytes. In this simple medium, a majority of hepatocytes stimulated by epidermal growth factor plus insulin entered S phase and divided, with a normal (13 h) interval between DNA synthesis and cell division. The proliferative response did not require extra substratum or the presence of serum, even during cell isolation and plating.

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Year:  1988        PMID： 3053597     DOI： 10.1007/BF02620869

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol        ISSN： 0883-8364


  28 in total

1.  Induction of DNA synthesis in cultured rat hepatocytes through stimulation of alpha 1 adrenoreceptor by norepinephrine.

Authors:  J L Cruise; K A Houck; G K Michalopoulos
Journal:  Science       Date:  1985-02-15       Impact factor: 47.728

2.  Hormonal regulation of DNA synthesis in primary cultures of adult rat hepatocytes: action of glucagon.

Authors:  D L Friedman; T H Claus; S J Pilkis; G E Pine
Journal:  Exp Cell Res       Date:  1981-10       Impact factor: 3.905

3.  Increased effect of adrenaline on cyclic AMP formation and positive beta-adrenergic modulation of DNA-synthesis in regenerating hepatocytes.

Authors:  G Brønstad; T Christoffersen
Journal:  FEBS Lett       Date:  1980-10-20       Impact factor: 4.124

4.  Changes in hormone responsiveness and cyclic AMP metabolism in rat hepatocytes during primary culture and effects of supplementing the medium with insulin and dexamethasone.

Authors:  T Christoffersen; M Refsnes; G O Brønstad; E Ostby; J Huse; F Haffner; T E Sand; N H Hunt; O Sonne
Journal:  Eur J Biochem       Date:  1984-01-16

5.  Induction of mitosis in primary cultures of adult rat hepatocytes under serum-free conditions.

Authors:  K Hasegawa; K Watanabe; M Koga
Journal:  Biochem Biophys Res Commun       Date:  1982-01-15       Impact factor: 3.575

6.  Quantitative aspects of the effects of insulin, epidermal growth factor and dexamethasone on DNA synthesis in cultured adult rat hepatocytes.

Authors:  T E Sand; G Brønstad; V Digernes; A Killi; W Amara; M Refsnes; T Christoffersen
Journal:  Acta Endocrinol (Copenh)       Date:  1985-07

7.  Cell cycle parameters of adult rat hepatocytes in a defined medium. A note on the timing of nucleolar DNA replication.

Authors:  O K Vintermyr; S O Døskeland
Journal:  J Cell Physiol       Date:  1987-07       Impact factor: 6.384

8.  The control of DNA synthesis in primary cultures of hepatocytes from adult and young rats: interactions of extracellular matrix components, epidermal growth factor, and the cell cycle.

Authors:  A Tomomura; N Sawada; G L Sattler; H K Kleinman; H C Pitot
Journal:  J Cell Physiol       Date:  1987-02       Impact factor: 6.384

9.  Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes.

Authors:  R A Richman; T H Claus; S J Pilkis; D L Friedman
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

10.  The kinetics of cellular proliferation in regenerating liver.

Authors:  J I Fabrikant
Journal:  J Cell Biol       Date:  1968-03       Impact factor: 10.539
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  6 in total

1.  Isolation and characterization of biliary epithelial cells from rainbow trout liver.

Authors:  J B Blair; G K Ostrander; M R Miller; D E Hinton
Journal:  In Vitro Cell Dev Biol Anim       Date:  1995-11       Impact factor: 2.416

2.  Maintenance of liver function in long term culture of hepatocytes following in vitro or in vivo Ha-rasEJ transfection.

Authors:  M Fischbach; H W Cao; M Diez Ibanez; C Tsaconas; S Alouani; F Montandon; M el Baraka; P Padieu; M Dreano; M Chessebeuf-Padieu
Journal:  Cell Biol Toxicol       Date:  1991-10       Impact factor: 6.691

3.  CCAAT/enhancer-binding protein (C/EBP) immunoreactivity during rat liver carcinogenesis.

Authors:  E Skarpen; B Lindeman; G H Thoresen; M Låg; T Christoffersen; H S Huitfeldt
Journal:  Histochem Cell Biol       Date:  1995-10       Impact factor: 4.304

4.  Regulation of surface expression of high-affinity receptors for epidermal growth factor (EGF) in hepatocytes by hormones, differentiating agents, and phorbol ester.

Authors:  I P Gladhaug; M Refsnes; T Christoffersen
Journal:  Dig Dis Sci       Date:  1992-02       Impact factor: 3.199

5.  Growth-regulatory effects of glucagon, insulin, and epidermal growth factor in cultured hepatocytes. Temporal aspects and evidence for bidirectional control by cyclic AMP.

Authors:  T E Sand; G H Thoresen; M Refsnes; T Christoffersen
Journal:  Dig Dis Sci       Date:  1992-01       Impact factor: 3.199

6.  Colony isolation and secondary culture of fetal porcine hepatocytes on STO feeder cells.

Authors:  N C Talbot; V G Pursel; C E Rexroad; T J Caperna; A M Powell; R T Stone
Journal:  In Vitro Cell Dev Biol Anim       Date:  1994-12       Impact factor: 2.416
  6 in total

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