Literature DB >> 6337376

Chromatographic resolution and kinetic characterization of glucokinase from islets of Langerhans.

M D Meglasson, P T Burch, D K Berner, H Najafi, A P Vogin, F M Matschinsky.   

Abstract

Glucokinase (ATP:D-glucose 6-phosphotransferase, EC 2.7.1.2) from rat islets of Langerhans was partially purified by chromatography on DEAE-Cibacron blue F3GA agarose. The enzyme eluted in two separate peaks. Sigmoidal rate dependence was found with respect to glucose (Hill coefficient = 1.5) for both enzyme fractions. Km values for glucose were 5.7 mM for the major fraction and 4.5 mM for the minor fraction. Neither fraction phosphorylated GlcNAc. A GlcNAc kinase (ATP:2-acetamido-2-deoxy-D-glucose 6-phosphotransferase, EC 2.7.1.59)-enriched fraction, prepared by affinity chromatography on Sepharose-N-(6-aminohexanoyl)-GlcNAc, had a Km of 25 microM for GlcNAc. Islet tissue also contained hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) eluting in multiple peaks. The results are consistent with the concept that glucokinase serves as the glucose sensor of pancreatic beta cells.

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Year:  1983        PMID： 6337376      PMCID： PMC393314          DOI： 10.1073/pnas.80.1.85

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  25 in total

Review 1.  Regulation of glucokinase in liver.

Authors:  S Weinhouse
Journal:  Curr Top Cell Regul       Date:  1976

2.  Kinetics of rat liver glucokinase. Co-operative interactions with glucose at physiologically significant concentrations.

Authors:  A C Storer; A Cornish-Bowden
Journal:  Biochem J       Date:  1976-10-01       Impact factor: 3.857

3.  Kinetic evidence for a 'mnemonical' mechanism for rat liver glucokinase.

Authors:  A C Storer; A Cornish-Bowden
Journal:  Biochem J       Date:  1977-07-01       Impact factor: 3.857

4.  Sequential analysis of the releasing and fuel function of glucose in isolated perifused pancreatic islets.

Authors:  W S Zawalich; F M Matschinsky
Journal:  Endocrinology       Date:  1977-01       Impact factor: 4.736

Review 5.  Protein purification using immobilised triazine dyes.

Authors:  P D Dean; D H Watson
Journal:  J Chromatogr       Date:  1979-10-01

6.  The isolation and preliminary characterization of N-acetyl-D-glucosamine kinase from rat kidney and liver.

Authors:  M B Allen; D G Walker
Journal:  Biochem J       Date:  1980-03-01       Impact factor: 3.857

7.  The identification of extrahepatic "glucokinase" as N-acetylglucosamine kinase.

Authors:  J Davagnino; T Ureta
Journal:  J Biol Chem       Date:  1980-04-10       Impact factor: 5.157

8.  Effects of iodoacetate, mannoheptulose and 3-O-methyl glucose on the secretory function and metabolism of isolated pancreatic islets.

Authors:  W S Zawalich; A S Pagliara; F M Matschinsky
Journal:  Endocrinology       Date:  1977-05       Impact factor: 4.736

9.  Apparent 'glucokinase' activity in non-hepatic tissues due to N-acetyl-D-glucosamine kinase.

Authors:  M B Allen; J L Brockelbank; D G Walker
Journal:  Biochim Biophys Acta       Date:  1980-08-07

10.  Kinetic characterization of N-acetyl-D-glucosamine kinase from rat liver and kidney.

Authors:  M B Allen; D G Walker
Journal:  Biochem J       Date:  1980-03-01       Impact factor: 3.857
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  25 in total

1.  Differences in glucose recognition by individual rat pancreatic B cells are associated with intercellular differences in glucose-induced biosynthetic activity.

Authors:  R Kiekens; P In 't Veld; T Mahler; F Schuit; M Van De Winkel; D Pipeleers
Journal:  J Clin Invest       Date:  1992-01       Impact factor: 14.808

2.  Glucose dependent K+-channels in pancreatic beta-cells are regulated by intracellular ATP.

Authors:  P Rorsman; G Trube
Journal:  Pflugers Arch       Date:  1985-12       Impact factor: 3.657

3.  Alternative splicing of glucokinase mRNA in rat liver.

Authors:  D J Hayzer; P B Iynedjian
Journal:  Biochem J       Date:  1990-08-15       Impact factor: 3.857

4.  GCK-MODY diabetes associated with protein misfolding, cellular self-association and degradation.

Authors:  Maria Negahdar; Ingvild Aukrust; Bente B Johansson; Janne Molnes; Anders Molven; Franz M Matschinsky; Oddmund Søvik; Rohit N Kulkarni; Torgeir Flatmark; Pål Rasmus Njølstad; Lise Bjørkhaug
Journal:  Biochim Biophys Acta       Date:  2012-07-20

5.  Effects of glucose and glucagon on the fructose 2,6-bisphosphate content of pancreatic islets and purified pancreatic B-cells. A comparison with isolated hepatocytes.

Authors:  A Sener; E Van Schaftingen; M Van de Winkel; D G Pipeleers; F Malaisse-Lagae; W J Malaisse; H G Hers
Journal:  Biochem J       Date:  1984-08-01       Impact factor: 3.857

6.  Rat glucokinase gene: structure and regulation by insulin.

Authors:  M A Magnuson; T L Andreone; R L Printz; S Koch; D K Granner
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

7.  In situ glucose uptake and glucokinase activity of pancreatic islets in diabetic and obese rodents.

Authors:  Y Liang; S Bonner-Weir; Y J Wu; C D Berdanier; D K Berner; S Efrat; F M Matschinsky
Journal:  J Clin Invest       Date:  1994-06       Impact factor: 14.808

8.  Pancreatic beta cell line MIN6 exhibits characteristics of glucose metabolism and glucose-stimulated insulin secretion similar to those of normal islets.

Authors:  H Ishihara; T Asano; K Tsukuda; H Katagiri; K Inukai; M Anai; M Kikuchi; Y Yazaki; J I Miyazaki; Y Oka
Journal:  Diabetologia       Date:  1993-11       Impact factor: 10.122

9.  Hexokinase isoenzymes from the Novikoff hepatoma. Purification, kinetic and structural characterization, with emphasis on hexokinase C.

Authors:  J Radojković; T Ureta
Journal:  Biochem J       Date:  1987-03-15       Impact factor: 3.857

10.  Pancreatic beta cells cultured from individual preneoplastic foci in a multistage tumorigenesis pathway: a potentially general technique for isolating physiologically representative cell lines.

Authors:  F Radvanyi; S Christgau; S Baekkeskov; C Jolicoeur; D Hanahan
Journal:  Mol Cell Biol       Date:  1993-07       Impact factor: 4.272
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