Nucleotide sequence of the yeast alcohol dehydrogenase II gene.

The complete nucleotide sequence of the glucose-repressed alcohol dehydrogenase II gene (ADR2) from yeast has been established together with its 5'- and 3'-flanking regions. The limits of the gene have been determined by reverse transcriptase sequencing of the 5'-ends and by S1 nuclease mapping of the 3'-ends of the mature ADR2 mRNA. Comparison of the alcohol dehydrogenase I gene (ADC1) sequence (Bennetzen, J. L., and Hall, B.D. (1982) J. Biol. Chem. 257, 3018-3025) with that of ADR2 indicated four regions of sequence conservation in the 5'-flanking DNAs. One of these conserved regions contains the sequence TCAAG which may function as a yeast cap sequence. The coding sequence of ADR2 is 89% homologous with that of ADC1 and exhibits a bias in its codon utilization. Evidence is presented that the intergenic region at the 3'-end of the ADR2 gene is less than 550 base pairs.