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Literature DB >> 6331350

Molecular cloning of the genomes of poliovirus type 3 strains by the cDNA: RNA hybrid method.

G Stanway, R C Mountford, S D Cox, G C Schild, P D Minor, J W Almond.

Abstract

The genomes of two neurovirulent strains of poliovirus type 3, wild type P3/Leon/37 and a vaccine revertant P3/119/70, have been cloned in E. coli. The cDNA: RNA hybrid method used was efficient and may have wide applicability for cDNA cloning. Overlapping clones spanning the entire genome were obtained for each strain. These have been used to produce full-length DNA copies of the two genomes each within a single plasmid.

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Year: 1984 PMID： 6331350 DOI： 10.1007/bf01309297

Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574

24 in total

1. Markers of poliovirus strains isolated from cases temporally associated with the use of live poliovirus vaccine: report on a W.H.O. collaborative study.

Authors:
Journal: J Biol Stand Date: 1981

2. Cloned poliovirus complementary DNA is infectious in mammalian cells.

Authors: V R Racaniello; D Baltimore
Journal: Science Date: 1981-11-20 Impact factor: 47.728

3. High-efficiency cloning of full-length cDNA.

Authors: H Okayama; P Berg
Journal: Mol Cell Biol Date: 1982-02 Impact factor: 4.272

4. Nucleic acid sequence of the region of the genome encoding capsid protein VP1 of neurovirulent and attenuated type 3 polioviruses.

Authors: G Stanway; A J Cann; R Hauptmann; R C Mountford; L D Clarke; P Reeve; P D Minor; G C Schild; J W Almond
Journal: Eur J Biochem Date: 1983-10-03

5. Polyadenylic acid at the 3'-terminus of poliovirus RNA.

Authors: Y Yogo; E Wimmer
Journal: Proc Natl Acad Sci U S A Date: 1972-07 Impact factor: 11.205

6. Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins.

Authors: A J Cann; G Stanway; R Hauptmann; P D Minor; G C Schild; L D Clarke; R C Mountford; J W Almond
Journal: Nucleic Acids Res Date: 1983-03-11 Impact factor: 16.971

7. 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.

Authors: H Land; M Grez; H Hauser; W Lindenmaier; G Schütz
Journal: Nucleic Acids Res Date: 1981-05-25 Impact factor: 16.971

8. Comparative biochemical studies of type 3 poliovirus.

Authors: P D Minor
Journal: J Virol Date: 1980-04 Impact factor: 5.103

9. Multiple genetic changes can occur in the oral poliovaccines upon replication in humans.

Authors: O M Kew; B K Nottay; M H Hatch; J H Nakano; J F Obijeski
Journal: J Gen Virol Date: 1981-10 Impact factor: 3.891

10. Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.

Authors: A Nomoto; T Omata; H Toyoda; S Kuge; H Horie; Y Kataoka; Y Genba; Y Nakano; N Imura
Journal: Proc Natl Acad Sci U S A Date: 1982-10 Impact factor: 11.205

7 in total

1. An assembly defect as a result of an attenuating mutation in the capsid proteins of the poliovirus type 3 vaccine strain.

Authors: A J Macadam; G Ferguson; C Arnold; P D Minor
Journal: J Virol Date: 1991-10 Impact factor: 5.103

Molecular cloning of the genomes of poliovirus type 3 strains by the cDNA: RNA hybrid method.

1. Markers of poliovirus strains isolated from cases temporally associated with the use of live poliovirus vaccine: report on a W.H.O. collaborative study.

2. Cloned poliovirus complementary DNA is infectious in mammalian cells.

3. High-efficiency cloning of full-length cDNA.

4. Nucleic acid sequence of the region of the genome encoding capsid protein VP1 of neurovirulent and attenuated type 3 polioviruses.

5. Polyadenylic acid at the 3'-terminus of poliovirus RNA.

6. Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins.

7. 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.

8. Comparative biochemical studies of type 3 poliovirus.

9. Multiple genetic changes can occur in the oral poliovaccines upon replication in humans.

10. Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.

1. An assembly defect as a result of an attenuating mutation in the capsid proteins of the poliovirus type 3 vaccine strain.

2. Nucleotide sequence of a neurovirulent variant of the type 2 oral poliovirus vaccine.

3. Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine.

4. Construction of poliovirus intertypic recombinants by use of cDNA.

5. The complete nucleotide sequence of a common cold virus: human rhinovirus 14.

6. Reversion to neurovirulence of the live-attenuated Sabin type 3 oral poliovirus vaccine.

7. Genetically Thermo-Stabilised, Immunogenic Poliovirus Empty Capsids; a Strategy for Non-replicating Vaccines.