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Literature DB >> 6330744

DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12.

J Piette, H Nyunoya, C J Lusty, R Cunin, G Weyens, M Crabeel, D Charlier, N Glansdorff, A Piérard.

Abstract

The carAB operon of Escherichia coli K-12, which encodes the two subunits of carbamoyl-phosphate synthetase (glutamine hydrolyzing) [carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); EC 6.3.5.5], is cumulatively repressed by arginine and the pyrimidines. We describe the structure of the control region of carAB and the sequence of the carA gene. Nuclease S1 mapping experiments show that two adjacent tandem promoters within the carAB control region serve as initiation sites. The upstream promoter P1 is controlled by pyrimidines; the downstream promoter P2 is regulated by arginine. Attenuation control does not appear to be involved in the expression of carAB. A possible mechanism by which control at these promoters concurs to produce a cumulative pattern of repression is discussed. The translational start of carA is atypical; it consists of a UUG or AUU codon.

Entities: Chemical Gene Species

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Year: 1984 PMID： 6330744 PMCID： PMC345383 DOI： 10.1073/pnas.81.13.4134

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

42 in total

1. Identification of the UUG codon as a translational initiation codon in vivo.

Authors: J G Files; K Weber; C Coulondre; J H Miller
Journal: J Mol Biol Date: 1975-06-25 Impact factor: 5.469

2. Unusual location and function of the operator in the Escherichia coli galactose operon.

Authors: R DiLauro; T Taniguchi; R Musso; B de Crombrugghe
Journal: Nature Date: 1979-06-07 Impact factor: 49.962

3. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors: A J Berk; P A Sharp
Journal: Cell Date: 1977-11 Impact factor: 41.582

Review 4. Regulatory sequences involved in the promotion and termination of RNA transcription.

Authors: M Rosenberg; D Court
Journal: Annu Rev Genet Date: 1979 Impact factor: 16.830

5. Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coli. Effects on carbamoyl phosphate and pyrimidine biosynthesis and on uracil uptake.

Authors: A Piérard; N Glansdorff; J Yashphe
Journal: Mol Gen Genet Date: 1972

6. Control of the biosynthesis of carbamoyl phosphate in Escherichia coli.

Authors: A Piérard; N Glansdorff; M Mergeay; J M Wiame
Journal: J Mol Biol Date: 1965-11 Impact factor: 5.469

7. Use of gene cloning to determine polarity of an operon: genes carAB of Escherichia coli.

Authors: M Crabeel; D Charlier; G Weyens; A Feller; A Piérard; N Glansdorff
Journal: J Bacteriol Date: 1980-08 Impact factor: 3.490

8. Molecular basis for modulated regulation of gene expression in the arginine regulon of Escherichia coli K-12.

Authors: R Cunin; T Eckhardt; J Piette; A Boyen; A Piérard; N Glansdorff
Journal: Nucleic Acids Res Date: 1983-08-11 Impact factor: 16.971

9. The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.

Authors: H Nyunoya; C J Lusty
Journal: Proc Natl Acad Sci U S A Date: 1983-08 Impact factor: 11.205

10. RNA polymerase involvement in the regulation of expression of Salmonella typhimurium pyr genes. Isolation and characterization of a fluorouracil-resistant mutant with high, constitutive expression of the pyrB and pyrE genes due to a mutation in rpoBC.

Authors: K F Jensen; J Neuhard; L Schack
Journal: EMBO J Date: 1982 Impact factor: 11.598

52 in total

1. Analysis of cloned structural and regulatory genes for carbohydrate utilization in Pseudomonas aeruginosa PAO.

Authors: L Temple; S M Cuskey; R E Perkins; R C Bass; N M Morales; G E Christie; R H Olsen; P V Phibbs
Journal: J Bacteriol Date: 1990-11 Impact factor: 3.490

2. Mutational analysis of intervening sequences connecting the binding sites for integration host factor, PepA, PurR, and RNA polymerase in the control region of the Escherichia coli carAB operon, encoding carbamoylphosphate synthase.

Authors: Neel Devroede; Nadine Huysveld; Daniel Charlier
Journal: J Bacteriol Date: 2006-05 Impact factor: 3.490

DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12.

1. Identification of the UUG codon as a translational initiation codon in vivo.

2. Unusual location and function of the operator in the Escherichia coli galactose operon.

3. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Review 4. Regulatory sequences involved in the promotion and termination of RNA transcription.

5. Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coli. Effects on carbamoyl phosphate and pyrimidine biosynthesis and on uracil uptake.

6. Control of the biosynthesis of carbamoyl phosphate in Escherichia coli.

7. Use of gene cloning to determine polarity of an operon: genes carAB of Escherichia coli.

8. Molecular basis for modulated regulation of gene expression in the arginine regulon of Escherichia coli K-12.

9. The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.

10. RNA polymerase involvement in the regulation of expression of Salmonella typhimurium pyr genes. Isolation and characterization of a fluorouracil-resistant mutant with high, constitutive expression of the pyrB and pyrE genes due to a mutation in rpoBC.

1. Analysis of cloned structural and regulatory genes for carbohydrate utilization in Pseudomonas aeruginosa PAO.

2. Mutational analysis of intervening sequences connecting the binding sites for integration host factor, PepA, PurR, and RNA polymerase in the control region of the Escherichia coli carAB operon, encoding carbamoylphosphate synthase.

3. Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase.

4. Role of the purine repressor in the regulation of pyrimidine gene expression in Escherichia coli K-12.

5. A combined theoretical and experimental study of the ammonia tunnel in carbamoyl phosphate synthetase.

Review 6. Histidine biosynthetic pathway and genes: structure, regulation, and evolution.

Review 7. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Review 8. Linkage map of Escherichia coli K-12, edition 8.

9. Regulation of carAB expression in Escherichia coli occurs in part through UTP-sensitive reiterative transcription.

10. Delineation of two distinct regulatory domains in the 5' region of the nar operon of Escherichia coli.