Literature DB >> 1242069

Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment.

G Pontecorvo.   

Abstract

Polyethylene glycol (PEG) is known to promote fusion of plant protoplasts. Various adaptations of this treatment to mammalian, including human, cell cultures are reported here. PEG is very effective in producing hybrids capable of indefinite multiplication even in cases, such as early passage human skin fibroblasts and lymphocytes, known to be highly recalcitrant to other treatments.

Entities:  

Mesh：

Substances：

Year:  1975        PMID： 1242069     DOI： 10.1007/bf01538671

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  126 in total

1.  Guido Pontecorvo ("Ponte"), 1907-1999.

Authors:  B L Cohen
Journal:  Genetics       Date:  2000-02       Impact factor: 4.562

2.  Bacterial fusion assayed by a prophage complementation test.

Authors:  C Sanchez-Rivas; A J Garro
Journal:  J Bacteriol       Date:  1979-03       Impact factor: 3.490

3.  Formation of multilayer rosettes in phytohemagglutinin-stimulated lymphocytes: correlation with chromatin condensation conformation in premature chromosome condensation.

Authors:  H Thomou; C Sambani; P Kitsiou; G Politis
Journal:  Cytotechnology       Date:  1988-07       Impact factor: 2.058

4.  Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP7(B2) in virion infectivity.

Authors:  M Sarmiento; M Haffey; P G Spear
Journal:  J Virol       Date:  1979-03       Impact factor: 5.103

5.  Requirements for species-specific papovavirus DNA replication.

Authors:  E R Bennett; M Naujokas; J A Hassell
Journal:  J Virol       Date:  1989-12       Impact factor: 5.103

6.  Influence of polymer concentration and molecular weight and of enzymic glycocalyx modification on erythrocyte interaction in dextran solutions.

Authors:  A J Baker; W T Coakley; D Gallez
Journal:  Eur Biophys J       Date:  1993       Impact factor: 1.733

7.  Membrane ultrastructural changes during calcium phosphate-induced fusion of human erythrocyte ghosts.

Authors:  N Zakai; R G Kulka; A Loyter
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

8.  Introduction of a human X-6 translocation chromosome into a mouse teratocarcinoma: investigation of control of HLA-A, B, C expression.

Authors:  P N Goodfellow; G Banting; J Trowsdale; S Chambers; E Solomon
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

9.  Assignment of adenosine deaminase complexing protein (ADCP) gene(s) to human chromosome 2 in rodent-human somatic cell hybrids.

Authors:  E Herbschleb-Voogt; K H Grzeschik; P L Pearson; P Meera Khan
Journal:  Hum Genet       Date:  1981       Impact factor: 4.132

10.  Haploid genome reactivation and recovery by cell hybridization. Induction of DNA synthesis in spermatid nuclei.

Authors:  S M Elsevier; F H Ruddle
Journal:  Chromosoma       Date:  1976-07-08       Impact factor: 4.316
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.