Literature DB >> 6329722

The Ca2+-dependent slow K+ conductance in cultured rat muscle cells: characterization with apamin.

M Hugues, H Schmid, G Romey, D Duval, C Frelin, M Lazdunski.   

Abstract

The interaction of apamin, a bee venom neurotoxin, with rat skeletal muscle cell membranes has been followed using both an electrophysiological and a biochemical approach. Voltage-clamp analyses have shown that apamin, at low concentrations, specifically blocks the Ca2+-dependent slow K+ conductance in rat myotubes and myosacs . A specific binding site for apamin in rat muscle cell membranes has been characterized with the use of a highly radiolabelled apamin derivative [( 125I]apamin). The dissociation constant for the apamin-receptor complex is 36-60 pM and the maximal binding capacity is 3.5 fmol/mg of protein. [125I]Apamin binding to rat muscle membranes is displaced by quinine and quinidine with K0.5 values of 110 microM and 200 microM, respectively.

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Year:  1982        PMID： 6329722      PMCID： PMC553159          DOI： 10.1002/j.1460-2075.1982.tb01293.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  19 in total

1.  The peptide components of bee venom.

Authors:  J Gauldie; J M Hanson; F D Rumjanek; R A Shipolini; C A Vernon
Journal:  Eur J Biochem       Date:  1976-01-15

2.  Mechanism of action of quinidine on squid axon membranes.

Authors:  J Z Yeh; T Narahashi
Journal:  J Pharmacol Exp Ther       Date:  1976-01       Impact factor: 4.030

Review 3.  Calcium-dependent potassium activation in nervous tissues.

Authors:  R W Meech
Journal:  Annu Rev Biophys Bioeng       Date:  1978

4.  Determination of protein: a modification of the Lowry method that gives a linear photometric response.

Authors:  E F Hartree
Journal:  Anal Biochem       Date:  1972-08       Impact factor: 3.365

5.  Apamin as a selective blocker of the calcium-dependent potassium channel in neuroblastoma cells: voltage-clamp and biochemical characterization of the toxin receptor.

Authors:  M Hugues; G Romey; D Duval; J P Vincent; M Lazdunski
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

6.  Ontogeny of the (Na+,K+)-ATPase during chick skeletal myogenesis.

Authors:  P Vigne; C Frelin; M Lazdunski
Journal:  J Biol Chem       Date:  1982-05-25       Impact factor: 5.157

7.  Single channel recordings of Ca2+-activated K+ currents in rat muscle cell culture.

Authors:  B S Pallotta; K L Magleby; J N Barrett
Journal:  Nature       Date:  1981-10-08       Impact factor: 49.962

8.  Na+ channels with binding sites of high and low affinity for tetrodotoxin in different excitable and non-excitable cells.

Authors:  A Lombet; C Frelin; J F Renaud; M Lazdunski
Journal:  Eur J Biochem       Date:  1982-05

9.  Specific binding and pharmacological interactions of apamin, the neurotoxin from bee venom, with guinea pig colon.

Authors:  M Hugues; D Duval; H Schmid; P Kitabgi; M Lazdunski; J P Vincent
Journal:  Life Sci       Date:  1982-08-02       Impact factor: 5.037

10.  Preparation of a pure monoiodo derivative of the bee venom neurotoxin apamin and its binding properties to rat brain synaptosomes.

Authors:  M Hugues; D Duval; P Kitabgi; M Lazdunski; J P Vincent
Journal:  J Biol Chem       Date:  1982-03-25       Impact factor: 5.157
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  23 in total

Review 1.  A review of the electrophysiological, pharmacological and single channel properties of heart ventricle muscle cells in the snail Lymnaea stagnalis.

Authors:  B L Brezden; D R Gardner
Journal:  Experientia       Date:  1992-09-15

Review 2.  Use of toxins to study potassium channels.

Authors:  M L Garcia; A Galvez; M Garcia-Calvo; V F King; J Vazquez; G J Kaczorowski
Journal:  J Bioenerg Biomembr       Date:  1991-08       Impact factor: 2.945

3.  Bay K 8644 enhances slow inward and outward currents in voltage-clamped frog skeletal muscle fibres.

Authors:  C Cognard; F Traoré; D Potreau; G Raymond
Journal:  Pflugers Arch       Date:  1986-12       Impact factor: 3.657

4.  The apamin-sensitive potassium current in frog skeletal muscle: its dependence on the extracellular calcium and sensitivity to calcium channel blockers.

Authors:  F Traoré; C Cognard; D Potreau; G Raymond
Journal:  Pflugers Arch       Date:  1986-08       Impact factor: 3.657

Review 5.  K+ channel modulators for the treatment of neurological disorders and autoimmune diseases.

Authors:  Heike Wulff; Boris S Zhorov
Journal:  Chem Rev       Date:  2008-05       Impact factor: 60.622

6.  Activation of a slow outward current by the calcium released during contraction of cultured rat skeletal muscle cells.

Authors:  B Constantin; C Cognard; M Rivet-Bastide; G Raymond
Journal:  Pflugers Arch       Date:  1993-05       Impact factor: 3.657

7.  Indolizinsulphones. A class of blockers with dual but discriminative effects on L-type Ca2+ channel activity and excitation-contraction coupling in skeletal muscle.

Authors:  P Bois; G Romey; M Lazdunski
Journal:  Pflugers Arch       Date:  1991-12       Impact factor: 3.657

8.  Characterization of apamin-sensitive Ca(2+)-activated potassium channels in human leukaemic T lymphocytes.

Authors:  C Hanselmann; S Grissmer
Journal:  J Physiol       Date:  1996-11-01       Impact factor: 5.182

9.  Characterization of apamin-binding protein associated with a Ca2+ -activated K+ channel.

Authors:  B Marquèze; M J Seagar; F Couraud
Journal:  J Protein Chem       Date:  1989-06

10.  The riluzole derivative 2-amino-6-trifluoromethylthio-benzothiazole (SKA-19), a mixed KCa2 activator and NaV blocker, is a potent novel anticonvulsant.

Authors:  Nichole Coleman; Hai M Nguyen; Zhengyu Cao; Brandon M Brown; David Paul Jenkins; Dorota Zolkowska; Yi-Je Chen; Brian S Tanaka; Alan L Goldin; Michael A Rogawski; Isaac N Pessah; Heike Wulff
Journal:  Neurotherapeutics       Date:  2015-01       Impact factor: 7.620
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