| Literature DB >> 6328307 |
C A Frederick, J Grable, M Melia, C Samudzi, L Jen-Jacobson, B C Wang, P Greene, H W Boyer, J M Rosenberg.
Abstract
The 3 A electron density map of a co-crystalline recognition complex between EcoRI endonuclease and the oligonucleotide TCGCGAATTCGCG reveals that a tight, complementary interface between the enzyme and the major groove of the DNA is the major determinant of sequence specificity. The DNA contains a torsional kink and other departures from the B conformation which unwind the DNA and thereby widen the major groove in the recognition site.Entities:
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Year: 1984 PMID： 6328307 DOI： 10.1038/309327a0
Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962